EpiCypher provides cutting edge tools and services for epigenetics and chromatin biology research. They pioneered the manufacture of designer recombinant nucleosomes that contain defined post-translational modifications. Their latest product, SNAP-ChIP, is the gold standard for antibody validation for chromatin IP and also serves as the most reproducible spike-in for normalizing ChIP and ChIP-seq assays. EpiCypher also develops assays for chromatin-modifying enzymes and post-translational modification binding proteins using a variety of readouts, such as Alpha, FRET and radioactivity, employing nucleosome or peptide-based substrates.
Nucleosomes are the physiological target of readers, writers and erasers that interact with or modify chromatin. The incorporation of nucleosome substrates into drug discovery assays is a dramatic improvement over peptides, providing access to historically challenging targets. A large portfolio of fully defined and homogeneous recombinant nucleosomes incorporating different DNA and histone modifications, site mutations, and histone variants is offered.
SNAP-ChIP uses DNA-barcoded recombinant designer nucleosomes (dNucs) as next-generation spike-ins for chromatin immunoprecipitation (ChIP). The SNAP-ChIP K-MetStat Panel consists of a panel of dNucs carrying well-studied histone lysine methyl marks.
EpiCypher has embarked on a massive effort to identify ChIP-capable reagents using Sample Normalization and Antibody Profiling (SNAP-ChIP), their proprietary recombinant nucleosome spike-in control technology.
Each of the SNAP-ChIP Certified Antibodies exhibits exquisite in-application performance to deliver reliable ChIP data that you can trust.
Chromatin remodeling regulates DNA access on chromatin and thus impacts gene expression and genome repair. EpiDyne nucleosome substrates are available for two readouts: Restriction Enzyme Accessibility (REA) and FRET. EpiDyne assays can be used to target SWI/SNF family remodeling complexes (e.g. SMARCA2 or SMARCA4) which are highly associated with numerous types of cancer.
EpiCypher’s EpiTriton Histone Peptide Array platform presents unmatched quality and diversity, with 296 peptides covering single and combinatorial modifications on histone H3, H4, H2A, or H2B. Biotinylated histone peptides are also available for purchase.