Mpox / Monkeypox
Browse BioCat's offering of Mpox research products including sample collection & preservation tubes, nucleic acid isolation kits, real-time PCR detection kits, recombinant proteins, ELISA assays and protein arrays.
There is a multi-country outbreak of the Mpox virus (formerly known as monkeypox) in several regions of the world, the full extent and impact of which remains unclear. While usually endemic to the areas of central and west Africa, the virus has recently spread to over 48 countries since being first identified in non-endemic regions. Currently the spread is believed to be minimal, the WHO, CDC, and other government entities are monitoring the situation closely.
The escape from the endemic regions has the World Health Organization on alert given the lack of associated travel within the region of those suspected and confirmed cases. Understanding how the virus is being spread, the populations it is spreading to, and the potential immune responses against the virus are of increasing importance in the research field.
Mpox Detection
How is Mpox detected? According to the World Health Organization, DNA polymerase chain reaction (PCR) is the preferred method of laboratory testing. The best samples are ones taken directly from the rash, whether it is a skin sample, fluid, or crust sample that is collected by vigorous swabbing. If skin lesions are not present, anal, rectal, or oropharyngeal swabs can be used.
However, PCR cannot be used for detection after the infection has cleared. Therefore, other techniques that rely on the host’s immune response to the virus are being used, including enzyme-linked immunosorbent assays (ELISA) that use subtle antigenic differences between viruses for detection.
Tools for Mpox Research
BioCat offers kits for the complete Mpox virus (MPXV) DNA detection workflow, covering all steps from sample collection to PCR detection. Tools for monitoring the immune response to MPXV, including ELISA Kits and Protein Arrays, are also provided.
- Sample Collection & Preservation Tubes
Safe transport and storage at ambient temperature - Nucleic Acid Isolation Kits
High-quality virus DNA from swab samples - Real-time PCR Detection Kits
Sensitive detection of MPXV-specific genes - ELISA Kits
Antibody screening to detect humoral immune response - Protein Arrays
11 crucial Mpox virus proteins to detect IgG, IgM, IgA response - Recombinant Proteins
Unique, high-purity Mpox virus proteins
Swab Collection & Nucleic Acid Preservation Tubes
The tubes are designed for swab DNA and RNA collection, especially for viral DNA or RNA isolation and analysis as not only the host nucleic acids but also all viral DNA/RNA present is preserved. Additionally, the tubes preserve DNA at room temperature for up to 4 months and RNA at room temperature for up to 2 months.
Isolation of Viral DNA from Swab Samples
High-quality total DNA from fresh and preserved swab samples.
Protocol for the isolation of DNA from swab samples using the Saliva DNA Isolation Kit.
Mpox Detection by Real-Time RT-PCR
Ready-to-use PCR assays for the detection of Mpox virus (MPXV) DNA utilizing two sets of specific primers and probes at the same fluorescent channel to detect the J2L and B6R genes of MPXV to enhance sensitivity. The 1-Step Nucleic Acid Stabilization Buffer included in the Monkeypox Virus 1-Step High Throughput PCR Kit eliminates the need for DNA extraction and purification steps.
The Mpox Virus Clade I & II PCR Nucleic Acid Detection Kit uses two sets of specific primers and probes in different fluorescent channels to detect the C3L gene for Clade I and the G2R gene for Clade II.
Mpox Human ELISA Kits
The Monkeypox (MPXV) Human ELISA Kits are in vitro indirect ELISAs for the semi-quantitative measurement of human IgA, IgG, or IgM (IgA/G/M) antibodies against MPXV antigen(s) in human serum or plasma. By quantifying the antibody within a sample, the humoral immune response and exposure to MPXV can be assessed. These ELISA kits are for research use only, not for therapeutic or diagnostic applications.
Mpox Recombinant Proteins
Highly pure recombinant proteins expressed in E. coli or HEK293 cells and containing a His-tag are available for many unique Mpox virus proteins.
Mpox Protein Arrays
The Mpox Virus Protein Arrays are semi-quantitative arrays displaying 11 important Mpox virus proteins, such as A29L, A30L, A33R, A35R, B16R, C19L, D6L, E8L, H3L, I1L, L1R, etc. They are suitable for IgG, IgM or IgA antibody detection in serum, plasma, and other liquid sample types.