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Sample Preparation

Sample Preparation Kits

Sample Collection & Preservation - DNA, RNA & Protein Purification

The unique suite of sample preparation kits provided by BioCat provides a fast, reliable and simple procedure for sample collection and preservation as well as for the subsequent purification of inhibitor-free RNA (including microRNA), DNA or proteins from various specimen types, including mammalian cells and (FFPE) tissues, blood, plasma/serum, saliva/swab, urine, stool, plants, fungi and soil. Multi-analyte kits allow for sequential or simultaneous isolation of RNA, DNA and proteins from the same sample using a single column with no sample splitting.

A large  variety of kits is especially designed for the purification of RNA and DNA, including cell-free circulating nucleic acids, from liquid biopsy samples including urine, plasma, serum and blood. Optimized preservation reagents grant the longest stability of DNA and RNA in e.g. urine, saliva and stool samples at ambient temperature.

In the majority of kits, purification is based on Norgen’s proprietary resin as the separation matrix. This unique patented silicon carbide resin allows rapid purification of RNA or DNA without the use of phenol or chloroform from a variety of samples. Due to the superior affinity of the resin even cell-free circulating RNA and DNA as well as exosomal RNA can be isolated from small sample amounts with high sensitivity.

The RNA and DNA are of the highest integrity and can be used in many downstream applications including real-time (RT) qPCR, methylation-sensitive PCR, next generation sequencing (NGS) and DNA array analysis.

A number of kits is available with CE-IVD marking in accordance with EU Directive 98/79/EC.