Super PiggyBac Transposase Expression Vector

Easy, consistent transgenesis with no limits on cargo size

Product Description

The Super PiggyBac transposase expression vector features a strong CMV promoter to provide robust expression of the transposase. The PiggyBac transposase coding sequence has been optimized for high expression, stability and integration activity in mammalian cells.

The Super PiggyBac Transposase recognizes transposon-specific inverted terminal repeats (ITRs) and efficiently integrates the ITRs and intervening DNA into the genome at TTAA sites. The Super PiggyBac Transposase is delivered to the cell via the Super PiggyBac Transposase Expression Vector, which is co-transfected with one or more PiggyBac Vectors.

The transposase plasmids are designed to use directly and cannot be propagated in bacteria.

Performance Data

Figure 1. Efficient transgenesis with the Super PiggyBac Transposase and both single- and dual-promoter PiggyBac Vectors. (Top four panels) Co-transfection with the Super PiggyBac Transposase Expression Vector and a Dual Promoter PiggyBac Cloning and Expression Vector (Cat.# PB513B-1-SBI) into HeLa cells demonstrates the efficient integration delivered by SBI’s PiggyBac Transposon System. After ten days of puromycin selection, only the cells co-transfected with the Super PiggyBac Transposase (+PB, right two panels) show robust growth and GFP fluorescence. (Bottom four panels) Co-transfection with the Super PiggyBac Transposase Expression Vector and a Single Promoter PiggyBac Cloning and Expression Vector (Cat.# PB531A-2-SBI) into HEK293 cells further demonstrates the efficient integration delivered by SBI’s PiggyBac Transposon System. After seven days of growth, the majority of cells that received the Super PiggyBac Transposase Expression Vector (+PB, right two panels) were RFP positive.

Customer Agreements

Academic customers may purchase PiggyBac Transposon System components for internal research purposes with no time restrictions, whereas commercial customers will be contacted after placing an order to sign a customer agreement granting a 12-month limited-use license (previously 6 months) to evaluate the technology.

SBI is fully licensed to distribute PiggyBac vectors as a partnership with Demeetra (formerly Hera BioLabs, Inc.).