PiggyBac Transposon System

Achieve easy, consistent transgenesis with no cargo size limits

• Cut and paste transposition
• One transfection makes transgenic cell lines
• Effective in human, mouse and rat cells
• No cargo limit — integrate 10-100kb
• All-in-one tight inducible PiggyBac vector

SBI’s PiggyBac Transposon System consists of a PiggyBac Vector and the Super PiggyBac Transposase which recognizes transposon-specific inverted terminal repeats (ITRs) and efficiently integrates the ITRs and intervening DNA into the genome at TTAA sites. The Super PiggyBac Transposase is delivered to the cell via the Super PiggyBac Transposase Expression Vector, which is co-transfected with one or more PiggyBac Vectors.

The PiggyBac Transposon System consists of two parts

1. PiggyBac transposase expression vector (PB210PA-1-SBI)
2. PiggyBac transposon vector of your choice

The PiggyBac all-in-one inducible vector (PBQM812A-1-SBI) will require the cumate solution (PBQM100A-1-SBI) for induction.

How it works

Figure 1. The PiggyBac Transposon System’s cut-and-paste mechanism.

• Step 1 The Super PiggyBac Transposase binds to specific inverted terminal repeats (ITRs) in the PiggyBac Cloning and Expression Vector and excises the ITRs and intervening DNA.
• Step 2 The Super PiggyBac Transposase inserts the ITR-Expression Cassette-ITR segment into the genome at TTAA sites.
  
  

Customer Agreements

Academic customers may purchase PiggyBac Transposon System components for internal research purposes with no time restrictions, whereas commercial customers will be contacted after placing an order to sign a customer agreement granting a 12-month limited-use license (previously 6 months) to evaluate the technology.

SBI is fully licensed to distribute PiggyBac vectors as a partnership with Demeetra (formerly Hera BioLabs, Inc.).