Mammalian Expression Systems

Find the solution you need for your mammalian-specific project

With a wealth of options for gene delivery and expression, SBI has the technologies, and gene expression vectors to support a wide variety of mammalian-specific projects.

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Enhanced Episomal Vectors (EEV) Technology

Nonviral, non-integrating technology
High levels of expression

The Enhanced Episomal Vectors (EEV) utilize nonviral, non-integrating plasmids and avoid the challenges of viral transduction systems. The technology is based on the Epstein-Barr Nuclear Antigen-1 (oriP-EBNA1) that has the ability to replicate in synchrony with the host genome by attaching to the host chromatin and replicating with each cell cycle division. This results in an extended presence within a host cell without integration or modification of the host´s genome.

Minicircle Technology

Episomal expression sustained over weeks
Foreign DNA-free

The episomal vectors of the Minicircle DNA Vector Technology are produced as circular expression cassettes devoid of any bacterial plasmid DNA backbone. Their smaller molecular size enables more efficient transfections and offers sustained expression over a period of weeks as compared to standard plasmid vectors that only work for a few days.

PiggyBac Transposon System

Random Integration
One transfection makes transgenic cell lines
No cargo limit — integrate 10-100kb

The powerful activity of the PiggyBac Transposon System enables genes of interest to be easily mobilized into target genomes. The PiggyBac (PB) transposon is a mobile genetic element that efficiently transposes between PiggyBac vectors and chromosomes via a "cut and paste" mechanism. The unique features of PiggyBac transposons are that there is no cargo limit and it is reversible. Transposons from the genome can be removed footprint-free.

PhiC31 Integrase Vector System

Site-specific integration
Unlimited insert size
Single-copy number

The PhiC31 Integrase System is an non-viral, integrase-based gene expression system. A variety of promoter and reporter combinations are available. The PhiC31 System is suitable for low- or single-copy integrations and supports integrations of large inserts without size constraints. It is frequently used for gene therapy development and related applications.

PinPoint Targeted Integration System

Site-specific integration
Unlimited insert size
Single-copy number

The PinPoint Targeted Integration System allows the easy and efficient production of isogenic stable cell lines in mammalian and other cell types. Custom gene expression cassettes can be engineered into target genomes using the unique PinPoint integrase with site-specific control. This technology enables the generation of platform cell lines which can be used to routinely knock-in different transgenes and reporters at the same genetic locus in cells with the same genetic background.

SpareQ Cumate Switch System

Turn expression on, off, then on again
Increase expression up to 40-fold
Tune expression levels

Get inducible gene expression with the SparQ Cumate Switch System through binding of cumate, a non-toxic small molecule, to CymR. Expression levels of your gene-of-interest are tightly controlled and increase with increasing cumate concentration until maximum induction is reached. Get as much as a 32-fold increase in gene expression with SparQ™, or a 40-fold increase with SparQ™2. Even better, induction is reversible, so you can turn expression levels on and off. Delivering negligible background expression in the absence of cumate, the SparQ System is an excellent choice for achieving controlled levels of gene expression.