PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector

Dual Promoter Expression Vectors for cDNA and microRNA

Product Description

This vector belongs to the PiggyBac Dual Promoter Vector Series. Vectors PB510B-1 to PB514B-2 carry the CMV promoter for high expression levels in most cell types, whereas vector PB713B-1 features the MSCV promoter which is active in stem cells.

The multiple cloning site (MCS) located downstream of the promoter allows for convenient cloning of your gene or microRNA of interest. Downstream of your expression cassette is an EF1alpha promoter driving the expression of either the Puro, GFP, RFP, GFP+Puro or RFP+Puro markers. The entire cassette is flanked by genomic insulator elements for stabilized expression and PiggyBac Inverted Terminal repeats for mobilization and integration.

The PiggyBac Transposon System consists of two parts

1. PiggyBac transposase expression vector (see below)
2. PiggyBac transposon vector of your choice

Related Products

Super PiggyBac Transposase Expression Vector

Customer Agreements

Academic customers may purchase PiggyBac Transposon System components for internal research purposes with no time restrictions, whereas commercial customers will be contacted after placing an order to sign a customer agreement granting a 12-month limited-use license (previously 6 months) to evaluate the technology.

SBI is fully licensed to distribute PiggyBac vectors as a partnership with Demeetra (formerly Hera BioLabs, Inc.).