SmartSEC-TC EV Isolation System

Product Description

The SmartSEC-TC Kit was specifically designed and optimized for use with large volumes of tissue culture media, including stem cells.

• Single Fraction Elution – no need to collect multiple fractions
• Higher yield than conventional SEC or silica resin-based isolation methods
• Scalable with minimal hands-on time (pour, spin, pipette… done!)
• High Quality and High Purity (retain native vesicular morphology with negligible BSA carryover)
• Suitable for diverse downstream applications: WB, NTA, TEM, RNA-Seq, proteomics or lipidomic profiling
• High Quality RNA Content

A Small Column for a Large Volume of Cell Media - The larger the sample volume, the longer it takes to extract pure and native EVs out of it? Not with our SmartSEC-TC. Building on the proven SmartSEC platform that powers our popular SmartSEC Single, SmartSEC HT Plate, and SmartSEC Mini Kit, SmartSEC-TC combines the efficiency and purity of size exclusion chromatography (SEC) with an advanced contaminant-trapping technology in a single 500µl fraction. Compared to both resin-based and bead-based methods, SSEC-TC delivers superior EV isolation results in terms of yield, purity, and overall integrity. The best part is the elimination of multi-fraction collection required with conventional SEC; therefore, delivering higher EV concentration. The kit includes an initial concentration step to reduce large tissue culture media volumes to a manageable size for downstream processing. Whether you are working on proteomics, genomics, or functional studies, SmartSEC-TC ensures that your EVs are ready for any analysis and application.

How it works

• Hands-on time: <10 minutes
• Total run time: <59 minutes

Figure 1. The simple SmartSEC-TC workflow.

Supporting Data

SmartSEC-TC Achieves Superior EV Purity Compared to Conventional SEC

Figure 2. SSEC-TC delivers higher yields of cleaner EVs compared to multiple pooled fractions obtained from conventional SEC (Company Q). To compare SmartSEC-TC with a competitor’s SEC-based columns, EVs were isolated from 10 mL of HEK293 cell culture media containing 10% exosome-depleted FBS (ExoFBS) using both methods. Western blot and fluorescent nanoparticle tracking analysis showed that SmartSEC-TC produced EVs with comparable particle concentrations but significantly higher EV marker proteins (CD63, CD9) and lower carryover proteins like BSA.

SmartSEC-TC is Your Go-To Solution for High-Yield and High-Purity EV Isolation from Serum-Free Media

Figure 3. Western blot analysis clearly demonstrates SSEC-TC outperforms a resin-based method (Company N). SmartSEC-TC efficiently isolates EVs from serum-free HEK293 cell culture media with high yield and purity, overcoming the challenges of reduced EV yield under serum-free conditions. EVs were isolated from 10 mL of conditioned media using SmartSEC-TC and Company N’s resin-based method. Western blot analysis revealed higher levels of EV marker proteins (CD9, CD81) and minimal BSA carry-over with SmartSEC-TC (A). Additionally, fNTA demonstrated over 6-fold higher particle concentrations and 3-fold greater purity with SmartSEC-TC compared to Company N (B).

SmartSEC-TC Ensures High-Purity EV Isolation in Low-Serum Stem Cell Culture

Figure 4. EVs isolated from PCS-500-011 adipose-derived mesenchymal stem cell culture media by SSEC-TC. EVs isolated from PCS-500-011 human adipose-derived mesenchymal stem cell culture media (containing 0.2% FBS) using SSEC-TC exhibited typical EV protein markers on Western blot (A) and demonstrated excellent EV particle yield as analyzed by fNTA. In addition, all the positive EV protein markers were validated using SBI’s Exo-Check Exosome Antibody Array assay (B).

SmartSEC-TC Ensures Morphological Integrity of Isolated EVs

Figure 5. SSEC-TC Isolated EVs Maintain Structural Authenticity. EVs isolated from HEK293 cell culture media using SmartSEC-TC exhibit typical EV morphology—intact vesicles with lipid bilayer membranes—and minimal visible background debris, as demonstrated by transmission electron microscopy (TEM).

EVs Isolated from SmartSEC-TC Contain High Quality RNAs

Figure 6. Small RNA quality assessment from EVs isolated using SmartSEC-TC from HeLa and HEK293 cell culture media (CCM). RNA samples were extracted using SBI’s EVery EV RNA Isolation Kit (Every100B-1) and analyzed with Agilent Bioanalyzer. The data showed excellent size distribution, minimal genomic DNA contamination, and high-quality small RNA enrichment within the 25-200 nt range.