SmartSEC Single EV Isolation System

Product Description

• Quick and easy isolation
• Better purity and yield than ultracentrifugation
• Higher EV concentration per fraction than conventional size exclusion chromatography kits
• Validated for human serum, plasma, and CSF as well as Aplysia californica hemolymph
• Compatible with most downstream applications such as mass spectrometry, western blotting, nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM) and RNA-sequencing (NGS)
  
  

SmartSEC Single uses the same proprietary chromatography-based extracellular vesicle (EV) isolation technology as our popular SmartSEC HT Plate Kit and SmartSEC Mini Kit but in a single tube format. SmartSEC technology combines all the benefits of size exclusion chromatography (SEC)—purity, yield, reproducibility, and preservation of EV integrity—with a contaminant trapping feature that enhances the capabilities of conventional SEC. The result is best-in-class EV isolation that is fast, easy, and clean.

How it works

The SmartSEC Single workflow is fast and easy. Simply apply 100 – 250 µl of cleared serum or plasma with additional column buffer or up to 4 mL of other biofluids directly to the pre-washed column, incubate, and centrifuge to elute the EVs.

Compatible with most downstream applications such as:

• Mass spectrometry
• Western blotting
• Nanoparticle tracking analysis (NTA)
• Transmission electron microscopy (TEM)
• RNA-sequencing (NGS)

Supporting Data

SmartSEC Single performs better than competitor’s q SEC technology

To understand how well SmartSEC Single performs compared to a competitor’s q SEC columns, we isolated EVs from 250 µL of human serum using both SmartSEC Single and q SEC columns and analyzed 1 µg of protein equivalent from each isolation method on a western blot (Figure 1) and using fluorescent nanoparticle tracking analysis (fNTA, Figure 2). EVs isolated using SmartSEC Single show lower levels of carry-over proteins such as albumin and IgGs (Figure 1) and a higher number of particles per mg of protein (Figure 2) than EVs isolated using the q SEC columns, demonstrating the higher purity delivered by SmartSEC Single.

In addition, SmartSEC Single delivered EVs at ~6-fold higher concentration than the q SEC columns (Figure 1), for overall superior performance.

Figure 1. Western blot analysis shows that SmartSEC Single delivers higher yields of cleaner EVs than a competitor’s q SEC columns.

Figure 2. fNTA show that SmartSEC Single delivers higher yields of cleaner EVs than a competitor’s q SEC column.

EVs isolated using SmartSEC Single possess typical EV morphology

Transmission electron microscopy (TEM) of EVs isolated from serum using SmartSEC Single possess typical EV morphology—intact vesicles with a double layer of membranes—with little visible background debris.

Figure 3. EVs isolated using SmartSEC Single possess typical EV morphology.