Isolation | Detection | Discovery | Services
Exosome Isolation at Exceptional Level of Purity and Yield
ExoQuick® ULTRA / ExoQuick®-TC ULTRA EV Isolation Kits for the purification of high-quality extracellular vesicles for downstream applications such as such as Western blotting, mass spectrometry, NGS sequencing, exosome labeling, and in vivo / ex vivo exosome delivery.
Peterson et al, 2015
Integrated systems for
Exosomes are 60 - 180 nm membrane vesicles secreted by most cell types in vivo and in vitro. These microvesicles are produced by the inward budding of multivesicular bodies (MVBs) and are released from the cell into the microenvironment following the fusion of MVBs with the plasma membrane. Exosomes are extracellular, nanoshuttle organelles that function as signal carriers and tissue reshapers through their cargo of RNA, proteins, and lipids, and are involved in a wide range of healthy and pathogenic processes including cancer, inflammation, immunity, CNS function, and cardiac cell function. They are found in blood, urine, amniotic fluid, breast milk, malignant ascites fluids and contain distinct subsets of microRNAs and proteins depending upon the cell type from which they are secreted, making them useful for biomarker discovery.
The next generation of exosomal/extracellular vesicle RNA isolation technology.
- Exosome RNA Next Generation Sequencing Service
- Exosome Mass Spectrometry
- Exosome Lipidomics and Metabolomics
- Exosome Nanosight NTA Analysis
Liquid Biopsy Analysis
Exosome Tools Overview
For gradient-based exosome isolation with increased yields and purity: ExoMAX™ Opti Enhancer takes you from biofluid to density gradient in just three easy steps - no preparatory ultracentrifugation step needed.
Apply ExoQuick® ULTRA / ExoQuick®-TC ULTRA EV Isolation Kits for the efficient isolation of highly pure extracellular vesicles for downstream applications like mass spectrometry, Western blotting of low abundance markers, exosome labeling, and in vivo / ex vivo exosome delivery.
Enrich exosomes for microRNA, mRNA and protein biomarker discovery in biofluids or tissue culture media using ExoQuick or ExoQuick-TC Exosome Precipitation Solution.
Simultaneously purify cfDNA and exosomes from serum or plasma for comprehensive biomarker research with XCF™ COMPLETE Exosome & cfDNA Isolation Kits.
Capture overall exosomes or exosome sub-populations from biofluids or cell culture media with ExoPure ImmunoBeads or ImmunoPlates.
Use ExoFACS Kits to isolate exosomes from biofluids or cell culture media and characterize exosomal proteins via fluorescence-activated cell sorting.
Use Exo-FBS and perform your studies of cellular secreted exosomes in culture without the worry of contaminating cow exosomes. Exo-FBS has been stripped of bovine exosomes yet supports robust growth of cells in culture.
In addition to ExoQuant Exosome Quantification Kits you can apply CD63-, CD9- or CD81-specific ExoELISA Kits to quantitate exosome particles. The EXOCET Exosome Quantitation Kit is a 96-well, plate-based colorimetric assay that works by detecting the exosomal acetecylcholinesterase (AChE).
Selectively capture distinct subpopulations of intact exosomes based on a particular surface marker and sort by FACS using Exo-Flow. Choose from tetraspanin, annexin, adhesion, fusion and immune presentation targets or customize your own capture system.
Confirm exosome recovery by Western Blot analysis with validated CD63, CD9, CD81 and Hsp70 antibodies or by using the Exo-Check Exosome Antibody Array containing well-characterized exosome protein markers.
Monitor exosome trafficking in real-time with exosomal Cyto-Tracers
Transfer siRNA, microRNA, mRNA, plasmid DNA and small molecule drugs directly into isolated exosomes for delivery into target cells using the Exo-Fect Exosome Transfection Kit
Exosome Component Labeling
Fluorescently label exosome RNAs, proteins, or membranes using ExoGlow Kits to visualize and track specific components in isolated EVs or to monitor exosome cargo delivery to cells in real-time.
Display proteins on exosome surfaces with XStamp Targeting Lentivectors and send them to specific destinations for cargo delivery.
RNA Packaging into Exosomes
Use the XMIR, AXMIR and XMIRXpress Exosome Packaging Systems to export miRNAs, anti-miRNAs and siRNAs of your choice into secreted exosomes by producer cells in culture.
Protein Packaging into Exosomes
Pack reporter proteins into exosomes to track cargo using XPack Cloning and Expression Lentivectors.
Exosomal microRNA Profiling
Isolate and profile exosomal RNAs from serum using the SeraMir Exosome RNA Amplification and Profiling Kits
Exosome RNA & DNA Purification
Apply Norgen Exosome Purification and RNA Isolation Kits to purify exosomes and isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA.
Extract and purify total RNA including small RNAs from immunocaptured exosomes with ExoRNA RNA Extraction Kits
Isolate circulating and exosome-associated genomic DNA from biofluids and cell culture media using ExoDNA DNA Extraction Kits
Exosome RNA-Seq and Mass Spec Sample Prep Kits
Discover exosome protein biomarkers by mass spec using the XPEP Mass Spec Peptide Library Sample Prep Kits capturing total protein or surface proteins.
An Exosome RNA Illumina NGS Sequencing and Analysis Service for the discovery of novel exosome-associated RNA biomarkers is also offered.
Use purified exosomes from human and mouse cell lines and purified exosomes from human biofluids for RNA, protein analysis, calibration standards and engineering cargo for delivery to target cells. All exosomes are characterized by NanoSight for size, intactness and concentration as well as tested to be exosome protein marker positive by Western Blot analysis.
Apply ExoStd Lyophilized Exosome Standards for multiple applications including FACS, WB, ELISA and as calibration standards for quantitation of exosome-derived markers from biological samples.