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Exosome Research

Isolation | Detection | Discovery | Services

NEW! XCF™ COMPLETE Exosome & cfDNA Isolation Kits

Simultaneous purification of cfDNA and exosomes from serum or plasma for comprehensive biomarker research.

Exosome Methods

Review Article:
Peterson et al, 2015
Integrated systems for
exosome investigation.

Exosomes are 60 - 180 nm membrane vesicles secreted by most cell types in vivo and in vitro. These microvesicles are produced by the inward budding of multivesicular bodies (MVBs) and are released from the cell into the microenvironment following the fusion of MVBs with the plasma membrane. Exosomes are extracellular, nanoshuttle organelles that function as signal carriers and tissue reshapers through their cargo of RNA, proteins, and lipids, and are involved in a wide range of healthy and pathogenic processes including cancer, inflammation, immunity, CNS function, and cardiac cell function. They are found in blood, urine, amniotic fluid, breast milk, malignant ascites fluids and contain distinct subsets of microRNAs and proteins depending upon the cell type from which they are secreted, making them useful for biomarker discovery.

Exosome Services

Liquid Biopsy Analysis

Kits for the enrichment and purification of circulating cell-free DNA and RNA as well as exosomal RNA for biomarker discovery.

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"Driving Research, Use and Engineering of Exosomes of Exosomes"


Exosome Tools Overview

ISOLATION DETECTION DISCOVERY ENGINEERING
NEW! ExoMAX™ Opti Enhancer
Gradient-based
Exo-Flow™ FACS Purified Exosomes Exo-Glow™ Cargo Labeling
ExoQuick® / ExoQuick-TC® Antibodies SeraMir™ XmiRNA Profiling Exo-Fect™ Transfection
Exo-Flow™ IP ExoCheck Ab Array XRNA Illumina® NGS Kits XMIRs miRNA Uploading Vectors
Exo-FBS™ Quantitation Kits & ELISAs XPEP Mass Spec Kits XPack Protein Packaging Vectors
      XStamp Exosome Targeting

Exosome Isolation

For gradient-based exosome isolation with increased yields and purity: NEW! ExoMAX™ Opti Enhancer takes you from biofluid to density gradient in just three easy steps - no preparatory ultracentrifugation step needed.

Enrich exosomes for microRNA, mRNA and protein biomarker discovery in biofluids or tissue culture media using ExoQuick or ExoQuick-TC Exosome Precipitation Solution.

Capture overall exosomes or exosome sub-populations from biofluids or cell culture media with ExoPure ImmunoBeads or ImmunoPlates.

Use ExoFACS Kits to isolate exosomes from biofluids or cell culture media and characterize exosomal proteins via fluorescence-activated cell sorting.

Exosome-depleted FBS

Use Exo-FBS and perform your studies of cellular secreted exosomes in culture without the worry of contaminating cow exosomes. Exo-FBS has been stripped of bovine exosomes yet supports robust growth of cells in culture.

Exosome Quantitation

In addition to ExoQuant Exosome Quantification Kits you can apply CD63-, CD9- or CD81-specific ExoELISA Kits to quantitate exosome particles. The EXOCET Exosome Quantitation Kit is a 96-well, plate-based colorimetric assay that works by detecting the exosomal acetecylcholinesterase (AChE).

Exosome Immunopurification

Selectively capture distinct subpopulations of intact exosomes based on a particular surface marker and sort by FACS using Exo-Flow. Choose from tetraspanin, annexin, adhesion, fusion and immune presentation targets or customize your own capture system.

Exosome Detection

Confirm exosome recovery by Western Blot analysis with validated CD63, CD9, CD81 and Hsp70 antibodies or by using the Exo-Check Exosome Antibody Array containing well-characterized exosome protein markers.

Exosome Tracking

Monitor exosome trafficking in real-time with exosomal Cyto-Tracers

Exosome Transfection

Transfer siRNA, microRNA, mRNA, plasmid DNA and small molecule drugs directly into isolated exosomes for delivery into target cells using the Exo-Fect Exosome Transfection Kit

Exosome Cargo Labeling

Fluorescently label endogenous exosome RNAs red and internal exosome proteins green with Exo-Glow Kits to monitor exosome cargo delivery to cells in real-time.

Exosome Targeting

Display proteins on exosome surfaces with XStamp Targeting Lentivectors and send them to specific destinations for cargo delivery.

RNA Packaging into Exosomes

Use the XMIR, AXMIR and XMIRXpress Exosome Packaging Systems to export miRNAs, anti-miRNAs and siRNAs of your choice into secreted exosomes by producer cells in culture.

Protein Packaging into Exosomes

Pack reporter proteins into exosomes to track cargo using XPack Cloning and Expression Lentivectors.

Exosomal microRNA Profiling

Isolate and profile exosomal RNAs from serum using the SeraMir Exosome RNA Amplification and Profiling Kits

Exosome RNA & DNA Purification

Apply Norgen Exosome Purification and RNA Isolation Kits to purify exosomes and isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA.

Extract and purify total RNA including small RNAs from immunocaptured exosomes with ExoRNA RNA Extraction Kits

Isolate circulating and exosome-associated genomic DNA from biofluids and cell culture media using ExoDNA DNA Extraction Kits

Exosome RNA-Seq and Mass Spec Sample Prep Kits

Apply XRNA Exosome RNA-Seq NGS Library Prep Kits specifically designed to make high-quality exosome RNA-Seq libraries for Illumina Next-Gen sequencing using low RNA input amounts. Discover exosome protein biomarkers using the XPEP Mass Spec Peptide Library Sample Prep Kit.

An Exosome RNA Illumina NGS Sequencing and Analysis Service for the discovery of novel exosome-associated RNA biomarkers is also offered.

Purified Exosomes

Use purified exosomes from human and mouse cell lines for RNA, protein analysis, calibration standards and engineering cargo for delivery to target cells. All exosomes are characterized by NanoSight for size, intactness and concentration as well as tested to be CD63 positive by Western Blot analysis.

Apply ExoStd Lyophilized Exosome Standards for multiple applications including FACS, WB, ELISA and as calibration standards for quantitation of exosome-derived markers from biological samples.

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