SmartSEC™ HT EV Isolation System (for Serum & Plasma)

Product Description

• High-throughput 96-well plate-based EV isolation
• Accelerate EV biomarker, diagnostics, and therapeutics research
• Isolate EVs from as many as 96 samples in less than one hour
• Use all the wells in a single run or or save wells for later use
• Load serum or plasma directly onto the SmartSEC HT plate after a brief centrifugation
• No need to pre-treat plasma
• Achieve levels of purity and yield better than ultracentrifugation
• Isolated EVs are compatible with most downstream applications
  
  

The SmartSEC HT EV Isolation System is a proprietary chromatography-based EV isolation method that comes in a 96-well plate format*. SmartSEC HT combines all the benefits of size exclusion chromatography (SEC)—purity, yield, reproducibility, and preservation of EV integrity—with a contaminant trapping feature that overcomes the limitations of conventional SEC for a fast, easy, and high-throughput EV isolation workflow.

*For processing individual samples we recommend using ExoQuick ULTRA which delivers similar purity and yields as SmartSEC™ HT but in a single reaction format.

Workflow

The SmartSEC HT workflow is fast, easy, and high-throughput (Figure 1). Simply prep the filter plate, apply 250 – 500 µL of cleared serum or plasma directly to each well, incubate, and centrifuge to elute the first fraction. Add an equal volume of SmartSEC Isolation Buffer and centrifuge again into a clean plate to elute the second fraction. Depending on the volume of sample loaded, the majority of EVs will be collected in either the first or second elution (we recommend collecting the two fractions separately for analysis and choosing to pool or not depending on your needs).  

Supporting Data

Figure 1. SmartSEC™ HT delivers high yields of EVs with low amounts of undesirable carry-over protein. EVs were prepared from 500 µL of serum (A) or plasma (B) using the indicated methods. For Western blot analysis, 1 µg protein equivalent from the first fraction was loaded into each lane. SmartSEC™ HT performs better than ultracentrifugation and a competitor’s “q” SEC column.

Figure 2. SmartSEC™ HT delivers higher yields than ultracentrifugation and a competitor’s “q” SEC column. We prepared EVs from 250 µL and 500 µL of serum and plasma using the indicated methods and determined yield using using (A) a Qubit protein assay and (B) fluorescent nanoparticle tracking analysis (fNTA). Table 1 provides a tabular summary of the plotted data values. By both EV yield methods, SmartSEC HT outperforms the other methods.