ExoQuick ULTRA EV Isolation Kit for Serum and Plasma

Uncompromising EV isolation delivers higher yields and cleaner preparations

Product Description

The revolutionary ExoQuick® ULTRA is SBI's most advanced polymer-based method for isolating Extracellular Vesicles, resulting in 420X more EVs than ultracentrifugation.

While many EV isolation methods require you to choose between high yields, easy protocols, clean preps, and low costs, ExoQuick ULTRA is able to deliver on all these fronts for trade-off free EV preparation.With our newest generation of ExoQuick—ExoQuick ULTRA—you no longer have to make the trade-off between yield, purity, speed, a simple protocol, and price. From as little as 250 μL of serum or plasma and our ExoQuick ULTRA kit, you can isolate high-quality EVs for a wide range of downstream applications.

To isolate exosomes from plasma, we recommend pre-treatment with the Thrombin Plasma Prep or using the ExoQuick™ Plasma Prep and Exosome Precipitation Kit. 

Features and Benefits

• Even cleaner—reduces carry-over of albumins by 75% and immunoglobulins by 40% more than other methods, including ultracentrifugation and competitors’ kits
• Higher yields—isolate more EVs per normalized input volume than ultracentrifugation and competitors’ kits
• Better biomarker detection—see what you’ve been missing when you increase the sensitivity of EV biomarker detection by up to 11-fold (CD9) and 8-fold (CD81)
• Fast—requires less than 20-minutes of hands-on time, including a convenient column-based format for clean-up
• Cost-effective—save money with each reaction compared to how much you’ll spend using a competitor’s kit
  
  

Questions?

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Applications

• Western blotting
• Mass spectrometry
• NGS sequencing
• Exosome labeling
• In vivo/ex vivo exosome delivery
• and more

How it works

The ExoQuick ULTRA protocol is quick and easy, requiring only 10-minutes of hands-on time: 

Figure 1. ExoQuick ULTRA Workflow

Each ExoQuick ULTRA Kit for Serum and Plasma comes with enough ExoQuick ULTRA (serum/plasma) reagent and convenient, pre-packed columns to complete 20 reactions. One reaction is defined as 250 μL of serum or plasma precipitated using ExoQuick ULTRA.

Performance Data

ExoQuick ULTRA vs. Top Competitor vs. Ultracentrifugation

Figure 2. ExoQuick ULTRA delivers high yields of clean exosomes. (A) A coomassie blue-stained protein gel comparing the protein content of exosome preps isolated using different methods shows only a few, defined protein bands in the ExoQuick ULTRA lane compared to the other methods. (B) Western blotting of the gel shows that the ExoQuick ULTRA prep contains the highest levels of exosome-specific markers CD9, CD81, and Hsp70 and the lowest levels of the carryover proteins albumin and IgGH. In contrast, the prep from Company Q appears to be primarily albumin, and even the sample prepared using ultracentrifugation contains considerably higher levels of both albumin and IgGH. Each lane was loaded with 7 ug of total protein as measured using a fluorometric Qubit protein assay.

Figure 3. Fluorescent nanoparticle tracking analysis (fNTA) demonstrates the high EV yields delivered by ExoQuick ULTRA compared to Ultracentrifugation. Comparison of different isolation methods on EV yields by both volume of input serum (per mL, A) and amount of input serum protein (per mg as measured by fluorometric Qubit protein assay, B). Particle number was measured using fNTA, a technique which specifically detects EVs (see how to use fNTA to measure EV concentration at our ExoGlow-NTA page).

Figure 4. EVs isolated using ExoQuick ULTRA display typical EV morphology. Transmission electron micrographs of EVs isolated from human serum using ExoQuick. The same sample is shown at two different magnifications. Multiple vesicles with typical EV morphology can be seen in each image.

More data of performance can be found in the ExoQuick ULTRA Product Sheet.

Any Questions Left?

Contact us for assistance to get started with the advanced polymer-based method for isolating Extracellular Vesicles.