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Cellecta

Cellecta

Cellecta, Inc., founded in 2006 is a custom and contract functional genomics solutions provider. Cellecta focuses primarily on developing and implementing flexible and scalable broad-based screening and analysis approaches for drug target and biomarker discovery. Their high-throughput functional genetic screening portfolio includes shRNA and CRISPR screening services, custom and off-the-shelf pooled libraries, and knockout/knockdown cell lines that facilitate genome-wide functional screening and the identification and validation of genes involved in critical biological and disease pathways.

The DriverMap Human Genome-Wide Expression Profiling Kit allows simultaneous profiling of all human protein-coding genes even from single cells in one reaction, thereby providing a reliable, accurate transcriptome profile with ultra-high sensitivity.


Cellecta Pooled Lentiviral Libraries Overview

Library Species Genes Modules Redundancy Notes
Genome-Wide CRISPR Human ~19,000
~19,000
1
3
4 sgRNAs (80K)
up to 8 sgRNAs (150K)
For knockout-based studies
HEAT sgRNA Design!
Mouse ~19,000 1 4 sgRNAs
Genome-Wide CRISPRa Human
Mouse
~19,000
~20,000
1 5 sgRNAs
Selective activation of targeted genes
HEAT sgRNA Design!
Genome-Wide CRISPRi Human
Mouse
~19,000
~20,000
1 5 sgRNAs
Selective inhibition of targeted genes
HEAT sgRNA Design!
Genome-Wide shRNA Human 19,276 3 8 shRNA Modules can be pooled for a single genome-wide experiment
Custom CRISPR
You decide You decide You decide You decide Fully customizable
HEAT sgRNA Design!
Custom shRNA You decide You decide You decide You decide Fully customizable
CloneTracker Barcodes ~50M barcodes 1 n/a Track individual clones from a population of cells by genomic DNA sequencing
CloneTracker XP RNA-expressed barcodes 1M barcodes
10M barcodes
10
1
n/a Track individual clones from a population of cells by genomic DNA sequencing and RNA-Seq

 

Cellecta Cas9 Expression Vectors

Cellecta´s Two Vector CRISPR/Cas9 System is used to perform functional knockout screens in vivo or in vitro by expressing sgRNA (single guide RNA, or gRNA) and Cas9 nuclease from different lentiviral vectors. The following Cas9 Expression Vectors with different resistance markers and GFP are offered.

Product Cat# Quantity
Cas9 Expression Construct Vector pR-CMV-Cas9-2A-Blast, Plasmid DNA SVC9B-PS-CT 25 ug
Cas9 Expression Construct pR-CMV-Cas9-2A-Blast, packaged lentiviral particles SVC9B-VS-GVO-CT 1 x 10e6 TU
Cas9 Expression Construct Vector pR-CMV-Cas9-2A-Hygro, Plasmid DNA SVC9-PS-CT 25 ug
Cas9 Expression Construct pR-CMV-Cas9-2A-Hygro, packaged lentiviral particles SVC9-VS-GVO-CT 1 x 10e6 TU
Cas9 Expression Construct Vector pR-CMV-Cas9-2A-Neo, Plasmid DNA SVC9N-PS-CT 25 ug
Cas9 Expression Construct pR-CMV-Cas9-2A-Neo, packaged lentiviral particles SVC9N-VS-GVO-CT 1 x 10e6 TU
Cas9 Expression Construct Vector pR-CMV-Cas9-2A-Puro, Plasmid DNA SVC9P-PS-CT 25 ug
Cas9 Expression Construct pR-CMV-Cas9-2A-Puro, packaged lentiviral particles SVC9P-VS-GVO-CT 1 x 10e6 TU
Cas9 Expression Construct Vector pR-CMV-Cas9-2A-GFP, Plasmid DNA SVC9G-PS-CT 25 ug
Cas9 Expression Construct pR-CMV-Cas9-2A-GFP, packaged lentiviral particles SVC9G-VS-GVO-CT 1 x 10e6 TU

 

CRISPR-Test Functional Cas9 Activity Assay Kits

Would you like to know if Cas9 is active in your cells? Cellecta’s CRISPR-Test™ assay kits accurately assess Cas9 activity in your cell line using two pre-mixed, lentiviral-packaged vectors:

  • one viral mix has a green fluorescent protein (GFP) lentivirus that responds to the specific Cas9 activity measured by the kit, whether nuclease, gene repression or gene activation
  • the other prepared lentiviral mix contains a red fluorescent protein (RFP) marker and functions as a control

Cas9 Nuclease
CRISPRuTest™ Functional Cas9 Activity Assay Kit for Any Cell (Mammalian)
CRISPRtest™ Functional Cas9 Activity Kit for Human Cells

dCas9-Activator
CRISPRaTest™ Functional dCas9-Activator Assay Kit

dCas9-Repressor
CRISPRiTest™ Functional dCas9-Repressor Assay Kit

CRISPRtest Workflow

CRISPRtest workflow.
A test population of cells is split into two wells. The responsive viral mix is introduced to one of the wells and the control virus to the other. After some days of incubation, cells from both cultures are analyzed by FACS. The ratio of GFP to RFP in the responsive population indicates the activity of the Cas9 variant being measured.

Publications

Browse the comprehensive list of publications from customers using Cellecta’s products and services, review articles, and other Cellecta research.