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Cellecta

Cellecta

Cellecta, Inc., founded in 2006 is a custom and contract functional genomics solutions provider. Cellecta focuses primarily on developing and implementing flexible and scalable broad-based screening and analysis approaches for drug target and biomarker discovery. Their high-throughput functional genetic screening portfolio includes shRNA and CRISPR screening services, custom and off-the-shelf pooled libraries, and knockout/knockdown cell lines that facilitate genome-wide functional screening and the identification and validation of genes involved in critical biological and pathological responses.

With the new Driver-Map Gene Expression and Molecular Profiling Service, Cellecta now offers a quantitative, multiplexed approach that allows simultaneous profiling of thousands of genes in one reaction, thereby providing the transcriptome profile of the tumor microenvironment.


Cellecta Pooled Lentiviral Libraries Overview

Library Species Genes Modules Redundancy Notes
Genome-Wide CRISPR Human only 19,001
19,001
1
3
4 sgRNAs
(80K)
up to 8 sgRNAs
(150K)
For knockout-based studies
HEAT sgRNA Design!
Genome-Wide shRNA Human only 19,276 3 8 shRNA Modules can be pooled for a single genome-wide experiment
DECIPHER (shRNA) Human 15,377 3 5+ shRNA Cannot pool Modules due to overlapping barcodes
Mouse 9,145 2 5+ shRNA
Custom CRISPR
Custom shRNA
You decide You decide You decide You decide Fully customizable
HEAT sgRNA Design!
CellTracker Barcode only >50M barcodes 1 n/a Track individual clones from a population of cells

 

Pooled Lentiviral Human Genome-Wide CRISPR sgRNA Library (80K)

The Pooled Lentiviral Human Genome-wide CRISPR sgRNA Library (80K) developed by Cellecta is the latest tool for performing loss-of-function screens. The CRISPR/Cas9 system can be used for permanent gene knockout by using a combination of sgRNA and a Cas9 nuclease. Target cells are transduced with Cas9-expressing lentivirus and selected to optimal Cas9 expression levels. The pooled sgRNA library is then transduced into cells so that a majority of the cells receives only one sgRNA construct. Loss-of-function screening followed by Next-Gen Sequencing (NGS) of integrated sgRNA from genomic DNA reveals which genes are involved in the biological process studied.

Cellecta’s Pooled Lentiviral Human Genome-wide CRISPR sgRNA Library (80K) was designed to be small enough (80,000 constructs) to enable convenient genome-wide “dropout viability” screens with most human cell lines. With 4 sgRNAs targeting each gene, all of which have been designed using the latest criteria for effective guides described by Doench et al. and containing the HEAT design sgRNA structure which Cellecta have proven to generate more robust screening results than the standard sgRNA design, the library has deep enough gene coverage to generate efficient results.

Pooled Lentiviral Human Genome-Wide CRISPR sgRNA Library (80K)

  • 1 single module, covering nearly all protein coding genes (over 19,000 gene targets)
  • Each gene is targeted by 4 sgRNAs for a total of 80,000 sgRNAs
  • sgRNA design based on Doench et al. Nat Biotechnol 2016 and containing the HEAT design sgRNA structure
  • Red fluorescence marker for sorting of transduced cells and puro selection marker
  • Compatible with Illumina HiSeq, GAIIx, and NextSeq NGS systems

A 3 Module Pooled Lentiviral Human Genome-Wide CRISPR sgRNA Library (150K) with up to 8 sgRNAs per target gene is also available, see table above.

 

Cellecta Cas9 Expression Vectors

Cellecta´s Two Vector CRISPR/Cas9 System is used to perform functional knockout screens in vivo or in vitro by expressing sgRNA (single guide RNA, or gRNA) and Cas9 nuclease from different lentiviral vectors. The following Cas9 Expression Vectors with hygromycin or blasticidin resistance marker are offered.

Product Cat# Quantity
Cas9 Expression Construct Vector pR-CMV-Cas9-2A-Hygro, Plasmid DNA SVC9-PS-CT 25 ug
Cas9 Expression Construct pR-CMV-Cas9-2A-Hygro, packaged lentiviral particles SVC9-VS-GVO-CT 1 x 10e6 TU
Cas9 Expression Construct Vector pR-CMV-Cas9-2A-Blast, Plasmid DNA SVC9B-PS-CT 25 ug
Cas9 Expression Construct pR-CMV-Cas9-2A-Blast, packaged lentiviral particles SVC9B-VS-GVO-CT 1 x 10e6 TU

 

Cellecta CRISPRtest Functional Cas9 Activity Kit

The CRISPRtest Functional Cas9 Activity Kit, currently for human cell lines only, is an easy-to-use, highly sensitive kit for measuring functional Cas9 activity in almost any human cell line. It is intended as a companion product for high-throughput CRISPR-based genetic screening or generation of isogenic knockout cell lines.

Publications

Browse the comprehensive list of publications from customers using Cellecta’s products and services, review articles, and other Cellecta research.