The PhiC31 Integrase System is an non-viral, integrase-based gene expression system for low- or single-copy integrations up to large insert sizes. The PhiC31 Systems comes in a variety of promoter and reporter combinations. Available are dual-direction promoter donor vectors as well as single promoter and promoterless options. The system is frequently used for gene therapy development and related applications.
With the PhiC31 Integrase System, you simply clone your gene-of-interest into the attB Donor Plasmid, and then (Step 1) co-transfect with a PhiC31 Integrase Expression Plasmid. (Inside the cell) The PhiC31 Integrase is transiently expressed, and mediates site-specific recombination between the attB site on the donor plasmid and a pseudo attP site in the genome. (Result) Because pseudo attP sites are typically present in transcriptionally active sites of the genome, your gene-of-interest will likely be integrated into an active region of the genome.
pFC-EF1-MCS- PGK-RFP-Puro vector
pFC-CMV-MCS-SV40-Kan/Neo phiC31 vector (single promoter)
pFC-CAG-MCS-WPRE-pA-SV40-Neo phiC31 vector (single promoter)
pFC-CMV-GFP-SV40-Kan/Neo phiC31 vector (positive control)
pFC-MCS-SV40-Kan/Neo phiC31 integrase vector (promoterless)
The phiC31 Integrase System can also be ulitized as a nonviral technology for transgenesis. With both iPSC-generating donor and PhiC31 Integrase Expression plasmids, the Non-Viral PhiC31 Integrase Mouse iPSC Reprogramming Kit delivers a complete, non-viral integrase-based iPSC generation system. The two plasmids are also available separately.