NEXTFLEX Cell Free DNA-Seq Library Preparation Kit

Liquid-biopsy and cell-free fetal DNA testing from as little as 10 ng input DNA in 3 hours

Product Description

Prepare cell-free DNA (cfDNA) libraries for sequencing in 3 hours with a kit optimized for fetal and circulating tumor DNA, compatible with Illumina® and Element® platforms. 

• Accelerate library preparation with a 3-hour workflow for cfDNA sequencing
• Simplify your protocol with a 1-step end-repair and adenylation process
• Enable high-throughput applications with up to 384 unique adapter barcodes
• Enhance variant detection in quantitative sequencing by integrating UDI-UMI barcodes
• Support research into fetal DNA and circulating tumor DNA applications

Product Details

Rapid and efficient library construction for cfDNA sequencing

The NEXTFLEX® Cell Free DNA-Seq Kit 2.0 is designed for the rapid construction of DNA libraries from cell-free DNA, specifically optimizing a 3-hour protocol for both fetal and circulating tumor DNA applications. This kit streamlines the preparation of single, paired-end, and multiplexed DNA libraries, ensuring broad compatibility with Illumina® and Element® sequencing platforms. This efficiency is critical for researchers investigating non-invasive biomarkers in clinical research settings.

Simplified 1-step workflow and high-throughput capabilities

A key feature of the NEXTFLEX® Cell Free DNA-Seq Kit 2.0 is its 1-step End-Repair and Adenylation protocol. This integration significantly shortens hands-on time during library construction, enhancing overall workflow efficiency. For studies requiring high sample volumes, the kit supports up to 384 unique adapter barcodes, enabling high-throughput applications without compromising data integrity or traceability.

Optimized for diverse clinical research applications

Cell-free DNA (cfDNA) has emerged as a valuable marker in clinical research. This kit is particularly suited for applications such as the detection of fetal DNA from maternal plasma for non-invasive prenatal research, including sex determination, and the identification of sex chromosome-linked disorders and aneuploidy events. Additionally, it supports research into circulating tumor DNA extracted from patient plasma, offering a non-invasive resource for investigating cancer diagnosis, prognosis, treatment responses, and follow-up monitoring.

For large cohort studies, pair the kit with up to 1,536 NEXTFLEX NGS Unique Dual Index (UDI) Barcodes to multiplex thousands of samples per flow cell with minimal index crosstalk.

Comprehensive kit components and optional nucleosome enrichment

The NEXTFLEX® Cell Free DNA-Seq Kit 2.0 contains all necessary reagents, except barcodes, to process purified cell-free DNA through the critical steps of library preparation: DNA End Repair & Adenylation, Adapter Ligation, and PCR Amplification. An optional Gel-Free Nucleosome Enrichment step is available to enrich for mono-, di-, and tri-nucleosomes, which can be performed prior to End Repair & Adenylation to optimize specific research outcomes. This kit is intended for research use only.

Compatibility and formats for various research scales

The kit is compatible with Illumina® and Element® sequencing platforms. It is supplied with sufficient materials to prepare either 8 or 48 Cell Free DNA samples, offering flexibility for studies of varying scales. For quantitative sequencing requiring the detection of low-frequency variants or rare somatic mutations, the use of NEXTFLEX UDI-UMI Barcodes is recommended in conjunction with this kit.

Streamlined Workflow

Figure 1: Sample flow chart with approximate times necessary for each step.

Performance Data

Figure 2: Example of final libraries ready for sequencing as appearing on the LabChip® GX Touch™ HT nucleic acid analyzer.

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