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NGS DNA Library Preparation Kits

NGS DNA Library Preparation Kits

Shorten your time to data with scalable true multiplexing library prep solutions

Accelerate your NGS workflows with a truly multiplexed library preparation solution engineered for speed, consistency and scalability. seqWell’s advanced tagmentation-based technology enables the rapid generation of high-quality libraries from hundreds to thousands of samples, dramatically reducing time to data.

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Tagmentation, the technique of simultaneous fragmenting and tagging (ligating NGS adapter sequences) of DNA is excellently suited to replace traditional library preparation methods that use complex and lengthy protocols that can limit the reach of NGS.

  • Workflow simplicity: Simultaneous DNA tagging (barcoding) and fragmenting streamlines NGS library preparation method
  • Tunability: Transposition reactions have defined stoichiometry - the number of events in a reaction can be controlled to produce a defined size and yield of library
  • Flexibility: Tagmentation can be utilized in a wide range of reaction conditions or sample types

 

Tagmentation

Tagmentation: Unfragmented genomic DNA is simultaneously cleaved and tagged using TnX transposase.

Tagmentation-based NGS DNA Library Preparation Kits

Our partner seqWell has developed highly innovative NGS DNA Library Preparation Kits which harness the power of tagmentation.  

  • Easier and faster workflows
  • High level of multiplexing
  • Automation-friendly
  • Significant cost and plastics savings
  • Auto-normalization of read counts and insert size over 10-fold input range
  • TnX: Next Generation Transposase with less GC bias and increased activity 

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ExpressPlex

The ExpressPlex 2.0 Library Preparation Kits are designed for rapid turnaround of plasmid, amplicon, or synthetic construct sequencing.They use the power of the Next Generation Transposase TnX for reduced bias and improved coverage uniformity. The TnX transposase-based workflow combines protocol simplicity with high-performance library preparation and generates sequence-ready libraries from extracted samples in less than half a day. The kits support automation and flexible reaction volumes.

AgriPrep

AgriPrep supports affordable, ultra-high-throughput plant & animal low-pass WGS & SKIM-sequencing approaches without sacrificing quality through unmatched protocol simplicity, auto-normalization, massive multiplexing, and lower all-in sequencing costs.  AgriPrep is available in bulk reagent formats and can be performed in ultra-high-throughput formats including 384-well or Echo-compatible plates.

MosaiX

MosaiX is a high-performance DNA library preparation kit that incorporates TnX into a novel directional tagmentation workflow. The workflow simplicity, the reduced insertion bias of TnX, and the performance benefits of ligation are combined to produce high complexity libraries.

Mosaixl Workflow Comparison Sqw

Long Plex

The LongPlex™ Long Fragment Multiplexing Kit fragments genomic DNA to the range of 7-10 kb while simultaneously adding barcodes in a rapid transposase-based enzymatic method. The initial fragmentation and barcoding steps take only ~35 minutes in a plate-based workflow. 

Tagify

Tagify™ loaded transposase reagents for tagmentation-based NGS library preparation enable simple, scalable, and reliable unique molecular identifier (UMI) additions or gene editing QC analysis. Choose between i5 UMI Adapter- or Custom-loaded transposase  and accelerate your variant calling or gene editing efforts with peace of mind.

PhiRx Indexed Control

The PhiRx™ Indexed Control is a color-balanced spike-in control for improved demultiplexing on XLEAP-SBS Illumina Sequencing Instruments.