NEXTFLEX NGS Library Normalization Beads

Product Description

NEXTFLEX™ NGS Library Normalization Beads capture a defined amount of library DNA during final cleanup to standardize input, promoting uniform cluster formation and balanced read counts across samples.

On-bead library normalization that equalizes sample molarity. Eliminates separate quantitation and manual pooling, reducing hands-on time and turnaround. Saves ~3 hours per 96-sample batch in high-throughput workflows. Optimized protocol reduces variability that can lead to over- or under-clustering. Can be seamlessly integrated into most library preparation protocols. Eliminates the capital expense of buying an instrument for normalization. Easily automated protocol can further reduce hands-on time. Not for use with PCR-free workflows.

Streamlined Workflow

Fig.1: Workflow comparison of post-library normalization vs on-bead normalization with NEXTFLEX Library Normalization Beads.

True Normalization

Fig.2: Normalization beads maintain an approximately constant recovered DNA concentration across a wide range of input DNA, demonstrating true normalization. In contrast, SPRI cleanup scales with input, increasing recovered DNA over the same range.