ExoQuick UltraPure EV Isolation Kit for Serum and Plasma

Achieve maximum yields of pure, apoB-free, intact EVs in just 20 minutes

Product Description

Why choose between yield and purity? With SBI’s ExoQuick® UltraPure, you get both. Our next-generation EV isolation kit combines proprietary precipitation with advanced mixed-mode spin-column technology to deliver higher yields, cleaner EVs, and reliable results in just 20 minutes—no ultracentrifuge required.

Features and Benefits

• Superior Recovery: Greater than 1000X higher yield vs. ultracentrifugation; >25× vs. SEC
• Cleaner Preps: ~96% ApoB removal and reduced albumin contamination
• High-Quality Cargo: Retains EV markers (CD9, CD81, TSG101) with consistent, BioAnalyzer-verified RNA
• Small Sample Ready: Works with 100–250 µL serum/plasma
• Fast, Simple Workflow: Pure EVs in 20 minutes, no ultracentrifuge or fraction collection
  
  

Questions?

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See how the ExoQuick UltraPure kit outperforms results from size exclusion chromatography (SEC) and ultracentrifugation. EV preparations isolated using the ExoQuick UltraPure kit surpass those isolated using traditional methods in every way: higher concentration of EVs, protein yield per milliliter, and overall yield and purity.

How it Works

Figure 1. The ExoQuick UltraPure workflow involves three basic steps: precipitation, purification, and collection.

Supporting Data

Figure 2. ExoQuick UltraPure Outperforms Other EV Isolation Methods. EVs were isolated from serum samples using ExoQuick UltraPure, the size-exclusion chromatography (SEC)-based method, and  ultracentrifugation (UC). (A) Total EV protein yield per mL of serum input was evaluated across ExoQuick UltraPure, SEC, and UC preparations. EV protein concentration was measured using a NanoDrop spectrophotometer. (B) Western blot analysis shows that ExoQuick UltraPure yields the highest levels of EV-specific markers (CD9, TSG101) and the lowest levels of albumin contamination. Each lane was loaded with an equal amount of EV protein. (C) Fluorescent nanoparticle tracking analysis (fNTA) demonstrates high EV yields delivered by ExoQuick UltraPure compared to ultracentrifugation and the SEC method.

Figure 3. ExoQuick UltraPure Enables More Efficient Removal of Lipoproteins. EVs were isolated from serum samples using the ExoQuick UItraPure kit, size-exclusion chromatography (SEC), and ultracentrifugation. (A and B) ApoB lipoprotein concentrations were measured by ELISA, revealing that ExoQuick UltraPure results in the lowest level of lipoprotein contamination.

Figure 4. High-Quality EV RNA. RNA isolated using the ExoQuick UltraPure kit and the EVery EV RNA isolation kit demonstrates excellent quality on Agilent Bioanalyzer profiles, enabling reliable downstream RNA analysis.

Any Questions Left?

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