EVery EV RNA Isolation Kit

Product Description

Obtain high yields of total EVery, including small RNAs, from already isolated EVs. The easy 30-min protocol delivers highly pure, highly concentrated RNA.

• Move quickly and confidently with exoRNA isolation that's high-yield and complete in <30 minutes
• Find what others miss when you capture every RNA with EVery
• Achieve phenol-level yields with a safer column-based method
• Get more RNA for each downstream reaction with EVery's small-volume elutions
• Ensure delivery of highly pure RNA by using the included DNaseI
• Compatible with most downstream applications, including RNA-seq and miRNA profiling
• Works with EVs isolated with commonly-used methods, such as ExoQuick, SmartSEC, and ultracentrifugation
• New column dimensions work just as smoothly for RNA isolation from all types of EV sample, irrespective of upstream EV isolation methods

EV samples vary drastically in their purity and concentration. Some EV samples from sera of diseased subjects and plasma bring up the challenge of heavy load of contaminating proteins that can potentially lead to column clogging thus lower yields. New column dimensions work just as smoothly for RNA isolation from all types of EV samples, irrespective of upstream EV isolation methods.

Overcoming many of the other challenges with RNA isolation from extracellular vesicles (EVs), the EVery EV RNA Isolation Kit is able to capture total EV RNA, including small RNAs. EVery is effective even with low amounts of input RNA and is capable of delivering high yields of highly pure RNA. Because the RNA elutes in a small sample volume, thus generating a highly concentrated prep, you can increase the amount of RNA used in a single downstream reaction for better data coverage quality.

How it works

The EVery EV RNA Isolation Kit delivers high yields of highly concentrated RNA from already isolated EVs. The column-based workflow is easy to implement and can be completed in less than 30 minutes

Figure 1. The quick and easy EVery EV RNA Isolation workflow.

The EVery EV RNA Isolation Kit can be used with EVs isolated by commonly used methods, including ExoQuick, SmartSEC, and ultracentrifugation, and comes with sufficient reagents to perform 20 purification reactions. The kit can also be bundled with SBI's  EVery cDNA Synthesis Kit, please ask for a quote.

Supporting Data

EVery captures EVerything

To demonstrate the ability of the EVery EV RNA Isolation Kit to capture the full range of RNAs, we used the kit to isolate RNA from 10,000 cells (Figure 2, lane 1), from EVs that were isolated from 250 µL of serum using SmartSEC Single (Figure 2, lane 2), and from buffer spiked with 0.1 pmol of Cel-miR-39 (Figure 2, lane 3). The high quality of the isolated RNA can be seen in lane 1, where the RNA integrity number (RIN) is 9.9 and the 28S/18S RNA ratio is 1.5.  The multiple bands in lane 2 demonstrate that EVery captures RNAs of different lengths—EVerything—from EVs with no apparent bias or size preference. The strong signal from the spiked-in miRNA in lane 3 demonstrates the good recovery of even small RNAs.

Figure 2. EVery captures EVerything.

EVery efficiently isolates mRNA

The EVery EV RNA Isolation kit was used to isolate mRNA from cells overexpressing eGFP (Figure 3). Robust levels of eGFP mRNA are recovered when cells are overexpressing eGFP.

Figure 3. EVery efficiently isolates longer RNAs like mRNA.