ExoQuick-TC Exosome Precipitation Solution

Isolate exosomes from tissue culture media fast and scalable

Product Description

ExoQuick-TC can be used to purify exosomes from a wide variety of tissue culture media, and from certain biofluids such as saliva, urine, follicular fluid, and breast milk. ExoQuick-TC is a proprietary polymer that gently precipitates exosomes.

With a simple workflow involving minimal hands-on time and low input sample volume requirements, ExoQuick-TC is an excellent option for researchers who need to purify multiple exosome samples.

Features and Benefits

• Saves time and labor
• Is easily scalable
• Conserves precious sample
• Delivers high yields of functional, high quality exosomes
• Can be used to isolate exosomes for a wide range of downstream applications
  
  

Questions?

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Applications

• Biomarker studies
• Exosomal miRNA profiling
• Exosomal proteomics and lipidomics/metabolomics
• Functional studies, such as in cell-to-cell signaling, basic biology, such as role in tumorigenesis

How it works

First, pre-clear your samples of cells and cellular debris, and then simply add the appropriate amount of ExoQuick-TC to your cleared biofluid, refrigerate, and centrifuge (see the product manual for protocol details). Your exosomes will be in the pellet, ready for resuspension in an appropriate solution.

To isolate exosomes from tissue culture media, simply:

1. Add an appropriate volume of ExoQuick-TC
2. Incubate overnight at 4°C
3. Isolate exosomes with a 30-minute low-speed spin (1500g).
   
   

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Supporting Data

Characterizing ExoQuick-TC exosomes with NanoSight

Exosomes purified with ExoQuick-TC from tissue culture media show the expected particle size distribution and high concentration yields when analyzed using NanoSight’s Nanoparticle Tracking Analysis (NTA, Figure 2).

Figure 2. Exosome size distribution and yields from tissue culture media. Cells from a human HT1080 lung sarcoma cell line were cultured in conditioned media (serum-free) for 72 hours. 10 mL of media was combined with 2 mL ExoQuick-TC, incubated overnight at 4°C, and centrifuged at 1500g for 30 minutes to isolate exosomes. The exosome pellet was resuspended in 1 mL PBS, diluted 1:40, and visualized on the NanoSight LM10 instrument. The analysis shows that ExoQuick-TC recovered 133 nm exosomes at a concentration of 1.74 x 10⁹ particles/mL. 

More data of performance can be found in the ExoQuick-TC Product Sheet.

Any Questions Left?

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