Mouse IL-23 Enhanced Episomal Expression Vector

Facilitate your studies of inflammation in mouse models with this EEV plasmid that provides constitutive, non-viral, sustained expression of the mouse IL-23 gene

Product Description

With virtually no limits on insert size (unlike AAV vectors) Enhanced Episomal Vectors (EEVs) are an excellent choice for non-integrating, non-viral gene expression. Because they replicate in synchrony with the host cell, they are stably inherited and can be used for long-lasting expression—up to several months both in vitro and in vivo—without modifying the host genome.

Get sustained, long-lasting, and non-viral expression of the mouse IL-23 gene from the  non-integrating CAGs-mIL-23 Enhanced Episomal Vector (EEV) vector. Driven by the constitutive CAG promoter, the CAGs-mIL-23 EEV works in most cell types, including primary cells and stem cells, and can even be directly injected into mice.

Performance Data

Sustained, non-integrating expression of mouse IL-23 in vivo via EEV

Figure 1. A constitutive EEV reporter based on CAGs-MCS leads to higher serum IL-23 levels in mice than two other episomal vector systems. SBI’s EEV system was compared to two other episomal vector systems—minicircle technology² and the mini-intronic plasmid system¹—using a mouse IL-23 cDNA cloned into the three different vectors. The three vectors or vehicle were introduced only into mice using hydrodynamic tail vein delivery (HDD). After seven days, serum IL-23 levels was measured using an ELISA assay and found that the EEV technology outperformed the other episomal platforms by at least 10-fold, demonstrating the ability of the EEV platform to provide sustained, long-lasting transgene expression.