PhiRx Indexed Control (10nM)

Reduce your rate of data loss from 53% to < 2%

Product Description

Maximize the performance of XLEAP-SBS chemistry on high-throughput Illumina platforms by color-balancing both the sequencing reads and barcodes using PhiRx Indexed Control.

• Optimizes index diversity for XLEAP sequencing
• Serves as a seamless spike-in sequencing control, similar to Illumina’s PhiX Control V3
• Corrects index color balancing issues for both low diversity barcodes and small batch sequencing runs
• Improves signal uniformity and data quality
• Helps recover data that would be lost as undetermined reads

PhiRx Indexed Control is a dual-indexed control library, made from phiX174 genomic DNA, optimized for Illumina sequencing platforms, particularly two-color systems like XLEAP SBS chemistry on NextSeq 1000/2000 and NovaSeq X. By improving color balancing for low-plexity libraries PhiRx ensures cleaner, more accurate sequencing results.

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Key Features

• 10 bp i5 and i7 indexes with mixed bases: Provides high base diversity, automatically meeting color balancing requirements on supported platforms. (patent-pending)
• Color balancing optimization: Specifically designed for the sensitive XLEAP SBS chemistry, enabling as little as 5% spike-in to correct base calling issues in libraries with low-diversity indexes.
• High diversity for low-plexity samples: Randomized inserts with 45% GC content improve sequencing accuracy for low-diversity libraries.
• Reliable quality control: Derived from the well-characterized phiX174 genome, providing robust calibration to support sequencing accuracy and performance.

PhiRx Indexed Control reduces the rate of data loss from 53% to < 2%

Table 1: A 96-plex library prepared using ExpressPlex 2.0 was sequenced on a NextSeq 2000 with 5% spike-in of Illumina PhiX Control V3 or PhiRx from seqWell.