Rap1 Activation Assay Kit

Product Description

Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways.

Rap, a 24 kDa protein of the Ras superfamily, regulates a variety of biological response pathways that include cell adhesion, proliferation, differentiation, and apoptosis.
The Ras-like proteins Rap1 and Rap2 share 60% identity. Like other small GTPases, Rap regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In their active (GTP-bound) state, Rap1 and Rap2 bind specifically to the Rap-binding domain (RBD) of RalGDS to control downstream signaling cascades.
Cell Biolabs’ Rap1 Activation Assay Kit utilizes RalGDS RBD Agarose beads to selectively isolate and pull-down the active form of Rap from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Rap is detected by western blot analysis using an anti-Rap1 polyclonal antibody.
Cell Biolabs’ Rap1 Activation Assay Kit provides a simple and fast tool to monitor the activation of Rap. The kit includes easily identifiable RalGDS RBD Agarose beads, pink in color, and a GTPase Immunoblot Positive Control for quick Rap1 identification. Each kit provides sufficient quantities to perform 20 assays.