ExoQuick-TC ULTRA EV Isolation Kit for Tissue Culture Media

Uncompromising EV isolation delivers higher yields and cleaner preparations

Product Description

Leveraging their years of exosome expertise, the SBI team has pushed ExoQuick-TC extracellular vesicle (EV) isolation technology to new peaks of performance with ExoQuick-TC ULTRA for tissue culture media and most biofluids.

While many EV isolation methods force you to choose between high yields, easy protocols, clean preps, and low costs, ExoQuick-TC ULTRA is able to deliver on all these fronts for trade-off free EV preparation. With our newest generation of ExoQuick-TC—ExoQuick-TC ULTRA—you no longer have to make the trade-off between yield, purity, speed, a simple protocol, and price. From as little as 5 mL of tissue culture media or other biofluid and our ExoQuick-TC ULTRA kit, you can isolate high-quality EVs for a wide range of downstream applications.

Features and Benefits

• Even cleaner—reduces carry-over of albumins by 75% and immunoglobulins by 40% more than other methods, including ultracentrifugation and competitors’ kits
• Higher yields—isolate more EVs per normalized input volume than ultracentrifugation and competitors’ kits
• Better biomarker detection—see what you’ve been missing when you increase the sensitivity of EV biomarker detection by up to 11-fold (CD9) and 8-fold (CD81)
• Fast—requires less than 20-minutes of hands-on time, including a convenient column-based format for clean-up
• Cost-effective—save money with each reaction compared to how much you’ll spend using a competitor’s kit

Questions?

Connect with us today to get started with your exosome isolation.

Applications

• Western blotting
• Mass spectrometry
• NGS sequencing
• Exosome labeling
• In vivo/ex vivo exosome delivery
• and more

How it works

The ExoQuick-TC ULTRA protocol is quick and easy, requiring only 10-minutes of hands-on time: 

Each ExoQuick-TC ULTRA Kit for tissue culture media and most fluids comes with enough ExoQuick-TC ULTRA reagent and convenient, pre-packed columns to complete 20 reactions. One reaction is defined as 5 mL of tissue culture media or other biofluid precipitated using ExoQuick-TC ULTRA

Performance Data

Figure 2. ExoQuick-TC ULTRA delivers high yields of clean exosomes. Comparison of different EV isolation methods via Western blot shows that the ExoQuick-TC ULTRA prep contains the highest levels of the exosome-specific marker CD63 and the lowest levels of the carryover protein albumin. In contrast, the prep from Company Q appears to be primarily albumin, and even the sample prepared using ultracentrifugation contains considerably higher levels of both albumin and IgGH. Each lane was loaded with 4 ug of total protein. 

Any Questions Left?

Contact us for assistance to get started with the most advanced polymer-based method for isolating Extracellular Vesicles.