Product Description

• Screen 182 exosomal miRNAs (in duplicates), carefully selected based on literature and in-house NGS data of miRNAs in serum/plasma exosomes
• Identify miRNA biomarkers and expression pattern signatures
• Compatible with various EV and EV-RNA isolation methods from SBI
• Use as little as nanogram amounts of starting EV RNA
• Conduct high-throughput screens of clinical samples
• Contains inbuilt controls for sample quality, monitoring hemolysis, isolation efficiency and interplate variation
• Primers dispensed and lyophilized in each well in an amount sufficient for one 10 µl reaction per well

Streamline your studies of miRNAs with this EVery miRNome Profiler for Human Serum and Plasma that contains the primers dispensed and lyophilized in each well in an amount sufficient for one 10 µl reaction per well. Each profiler plate includes primers for 182 miRNA (in duplicates), carefully selected based on literature and in-house NGS data of miRNAs in serum/plasma exosomes, as well as some candidate reference miRNAs (hsa-miR-103a-3p, hsa-miR-191-5p, hsa-miR-16-5p, hsa-miR-423-3p and hsa-let-7a-5p), from miR-451a and miR-23a to monitor hemolysis, 5 synthesized RNA spike-ins as control for protocol and IPC (inter plate control) for plate uniformity. All miRNA assays are based on miRBase entries.

From just 250 ul of serum or plasma one can assay for differential expression of preselected 182 exosomal miRNAs (in duplicates) between normal and diseased samples. This can be significant for biomarker discovery and validation, opening up potential for diagnosis and treatment monitoring.

EVery miRNome Profiler for Human Serum and Plasma has been extensively validated to work equally well with various upstream EV isolation (ExoQuick, ExoQuick-Ultra and SmartSEC and EV RNA isolation products (RA808A-1 and EVery100B-1) from SBI’s portfolio. Once you have already isolated EV RNA from your samples you may simply start with the EVery cDNA Synthesis Kit to make the cDNA template that is ready to go into qPCR with EVery miRNome Profiler for Human Serum and Plasma. Use the EVery miRNome Profiler with SBI’s new EVery miRNA Spike-in Kit that supplies separate Spike-in mixes for RNA isolation and cDNA synthesis steps covering high, medium and low abundance miRNAs as well as their Forward primer for qPCR.

How It Works

Using the EVery miRNome Profiler for Human Serum and Plasma

1. Prepare cDNA for qPCR using EVery cDNA Synthesis Kit, or other cDNA synthesis method of your choice
2. The result is a cDNA pool of anchor-tailed miRNAs that are ready for qPCR
3. Then conduct qPCR assays with EVery miRNome Profiler

Get additional information about the array layout and miRNA sequences, in this Document, “384-well plate layout for 2x EVery miRNome PCR Panel"

Supporting Data

Figure 1. Heat map of normal vs. breast cancer serum miRNA expression profiles Representative data normalized to global mean of each sample. Breast cancer patient serum showed distinct miRNA expression profiles compared to normal serum.

Figure 2. EVery miRNome Profiler identifies differential expression of selected miRNAs in normal vs. breast cancer serum. Data normalized with global mean. Expression of miR-451a, miR-21-5p, miR-16-5p and miR-103a are significantly upregulated, while expression of the tumor suppressor miR-124-3p is significantly downregulated in breast cancer serum compared to normal serum