EVery cDNA Synthesis Kit

Product Description

Get your EV RNA ready for downstream analysis with this quick and easy cDNA synthesis kit optimized for use with the EVery EV RNA Isolation Kit.

• Get quick (<2 hrs) and easy cDNA synthesis optimized for use with the EVery EV RNA Isolation Kit
• Find low abundance RNAs with the optional pre-amplification step
• Use with most downstream applications such as PCR, qPCR and miRNA profiling
• Includes sufficient Universal reverse primer for all 8 profiles from EVery miRNome Profiler for human Serum and Plasma

Optimized for use with the EVery EV RNA Isolation Kit, the EVery cDNA Synthesis Kit provides high-quality exosomal cDNA for your downstream applications such as PCR, qPCR, miRNA profiling, and RNA-seq. The kit generates cDNA through poly(A) tailing of exoRNA followed by reverse transcription with MMLV Reverse Transcriptase.

The optimized Universal reverse primer, included in the kit, will anneal efficiently to the adaptor sequence incorporated at the 3’ end of cDNA. This can be used for PCR amplification of desired RNA(s) along with specific forward primer(s) annealing to the RNA(s) of interest.

The EVery cDNA Synthesis Kit comes with sufficient reagents to perform 20 purification reactions. The kit can also be bundled with SBI's EVery EV RNA Isolation Kit, please ask for a quote.

Supporting Data

Robust cDNA synthesis with EVery cDNA Synthesis Kit

Figure 1. EVs were isolated from 250 µL of serum using SmartSEC Single, spiked in 0.1 pmol of Cel-miR-39. The EVery EV RNA Isolation Kit and a phenol-based kit were used to isolate RNA. The isolated RNA was reverse transcribed using the EVery cDNA Synthesis Kit and the copy number of Cel-miR-39 measured. The EVery EV RNA Isolation Kit delivered similar levels of Cel-miR-39 as the phenol-based method.

Figure 2. EVery EV RNA Isolation Kit and EVery cDNA Synthesis Kit were used to isolate mRNA and synthesize cDNA from cells overexpressing eGFP. Robust levels of eGFP mRNA were recovered and converted to cDNA when cells are overexpressing eGFP.

Figure 3. EVery family of products generate high-quality cDNA suitable for miRNA profiling.

Figure 4. Differential expression of selected miRNAs in normal vs. breast cancer serum confirmed by qPCR. Data normalized with global mean. Expression of miR-451a, miR-21-5p, miR-16-5p and miR-103a are significantly upregulated, while expression of the tumor suppressor miR-124-3p is significantly downregulated in breast cancer serum compared to normal serum.