OxiSelect™ BPDE DNA Adduct ELISA Kit
| Specifications | |
|---|---|
| Sample Type: | Suitable for use with DNA samples such as cell or tissue genomic DNA |
| Detection Method: | Colorimetric |
Product Description
The OxiSelect BPDE DNA Adduct ELISA Kit is an enzyme immunoassay developed for rapid detection of BPDE-DNA adducts. The quantity of BPDE adduct in DNA samples is determined by relative comparison of a known BPDE-DNA standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples.
Assay Principle
BPDE-DNA standards or unknown DNA samples are adsorbed onto a 96-well DNA high-binding plate. The BPDE-DNA adducts present in the sample or standard are probed with an Anti-BPDE-I Antibody, followed by an HRP Conjugated Secondary Antibody. The BPDE-DNA adduct content in an unknown sample is determined by comparing with a standard curve that is prepared from predetermined BPDE-DNA standards.
Product Citations
- Zeng JF, et al. (2018) miR-23b-3p and miR-125b-5p downregulate apo(a) expression by targeting Ets1 in HepG2 cells. Cell Biol In
- Beranek, M. et al. (2016) Genetic polymorphisms in biotransformation enzymes for benzo [a] pyrene and related levels of benzo [a] pyrene-7, 8-diol-9, 10-epoxide-DNA adducts in Goeckerman therapy. Toxicol Lett.
- Peters, R. E. et al. (2016) Do biomarkers of exposure and effect correlate with internal exposure to PAHs in swine?. Biomarkers.
- Yang, J. et al. (2016) Ubiquitin protein ligase Ring2 is involved in S-phase checkpoint and DNA damage in cells exposed to benzo [a] pyrene. J Biochem Mol Toxicol.
- Barhoumi, R. et al. (2014) Effects of fatty acids on benzo [a] pyrene uptake and metabolism in human lung adenocarcinoma A549 cells. PLoS One.
- Catalog Number
STA-357-CB - Supplier
Cell Biolabs - Size
- Shipping
Blue Ice
Price
962,00 €
