iLenti-GFP siRNA Cloning and Expression Vector (HIV based) (BbsI linearized)

Product Description

ABM’s iLenti-GFP expression system is an HIV lentiviral vector-based siRNA expression system with the following advantages:

• iLenti™ vectors are very stable and have the ability to grow in regular competent cells, which eliminates the need for special, stable competent cells
• iLenti™ uses convergent promoters to avoid hairpin loop structure design, thus cloned siRNA will be less likely to form secondary structures, allowing easier plasmid propagation and sequencing
• The convergent promoter design also enables longer siRNA designs of 27-29bp oligos, which have been shown to be more efficient than traditional 21mers in specific gene knockdown (Kim et al. 2005)
• iLenti™ significantly reduces the production cost of oligos, as ~33bp inserts are used instead of the >60bp oligos used in shRNA based systems
• The iLentiTM vector has a unique, single Bbs I restriction enzyme site in the multiple cloning site, which allows efficient, directional cloning of siRNA target sites
• Kanamycin / Neomycin Selection Marker
• GFP reporter gene incorporated under the CMV promoter allows simultaneous tracking and sorting of expressed siRNAs in vivo and FACS sorting of successfully transduced cells