ExoGlow-NTA Fluorescent Labeling Kit (for Malvern NanoSight)

Product Description

• Specifically label EVs for fluorescent NanoSight NTA quantitation
• Proprietary dye for high signal-to-noise ratio
• Compatible with common EV isolation methods including ExoQuick™, ultracentrifugation, and column-based methods
• 45-minute protocol from sample isolation to analysis

Making a great exosome research tool even better, SBI has developed ExoGlow-NTA Labeling Kit (for Malvern NanoSight), a proprietary dye that enables fluorescent NTA analysis of only the extracellular vesicles present in a heterogenous sample. The result is more accurate EV NTA data that excludes protein aggregates, membrane fractions, and other background particles to provide EV-specific particle size and concentration.

Gain more accurate insight into your exosome sample

The ExoGlow-NTA Fluorescent Labeling Kit (for Malvern NanoSight) takes advantage of the fluorescence capabilities of nanoparticle tracking analysis instrumentation with a proprietary fluorescent dye, which works by reacting specifically and efficiently to the surface of intact vesicles. Membrane fragments, protein aggregates, and other background particles do not activate the ExoGlow-NTA dye, resulting in exclusion of these species from fluorescent NTA analysis (Figure 1). Thus, with the ExoGlow-NTA Kit, the data delivered by NTA more accurately represents the EV populations in your sample rather than all particles, as is typically reported by conventional (non-fluorescent) NTA.

*For fluorescent NTA analysis, the NanoSight NTA system (model LM10, NS300 or others) MUST be installed and properly calibrated with a 488nm laser (available separately). Please contact your local Malvern Instruments representative for additional information.

Figure 1. The ExoGlow-NTA dye only binds to membranes of intact EVs. Unlike conventional NTA, which collects data on all particles in a solution based on light scattering, the fluorescence mode of the NanoSight instrument selectively detects the labeled EVs and only the data from fluorescently-labeled particles is reported.

Supporting Data

SBI’s ExoGlow-NTA Fluorescent Labeling Kit delivers highly accurate EV quantitation in a quick and easy workflow. The proprietary dye offers highly efficient labeling (Figure 2), very low background (Figure 3), and is compatible with a wide range of EV isolation techniques.

Figure 2. ExoGlow-NTA-labeled liposomes deliver consistent NanoSight NTA data whether in light scattering or fluorescent mode. The high concordance of NTA and fluorescent NTA data collected from the ExoGlow-NTA Kit internal standards (ExoGlow-NTA-labeled synthetic liposomes) demonstrates the labeling efficiency of the ExoGlow-NTA Dye and accuracy of the fluorescent NTA method for characterizing EVs.

Figure 3. ExoGlow-NTA delivers undetectable background signal. When analyzing the ExoGlow-NTA dye alone in PBS, conventional NTA picks up background particles in the absence of EVs, while fluorescent NTA of the ExoGlow-NTA dye alone shows bias-free undetectable autofluoresence, based on (A) particle counts and (B) imaging.

Kit Contents

The ExoGlow-NTA Fluorescent Labeling Kit comes with three components: 1) Labeling dye 2) Internal Standards and 3) Reaction Buffer. Simply mix the dye with the reaction buffer, add 1-100 µg of EVs (or protein equivalent), incubate for 30 minutes, and you are ready for fluorescent NTA analysis. The provided Internal Standards are size-controlled synthetic liposomes that provide a positive control for NanoSight calibration as well as EV/exosome labeling efficiency using the ExoGlow-NTA Kit.

Fluorescent NTA Analysis Service

Don't have access to a NanoSight instrument equipped with the 488nm laser? We also offer fluorescent NTA as a service, simply send us your samples.