Fluorescent NTA-based Exosome Characterization Service

Fast and efficient - SBI's NTA Analysis Service is an excellent way to characterize your exosome preps, with no need for your own NTA instrument

Product Description

How to order the NTA-based Exosome Characterization Service

• Contact us for a quote
• Send us your biofluid or exosome prep
• Get your data files in approximately 3 weeks

Additional costs for shipping your samples to our service partner might apply!

What’s NTA?

SBI uses NTA measurements that rely on light scattering to extract particle size and the number of particles in a sample (Figure 1A). Briefly, a laser is directed at the sample and the particles in the sample scatter the light, which is imaged by a camera attached to a microscope and operating at 30 frames per second. The NTA software collects data on multiple particles simultaneously, and calculates the hydrodynamic diameter of each particle using the Stokes-Einstein equation.

A. Conventional NTA

All particles are visible to NTA

B. Fluorescent NTA

Only ExoGlow-NTA-labeled particles are visible to NTA

Figure 1. The ExoGlow-NTA dye only binds to membranes of intact EVs. Unlike conventional NTA (A), which collects data on all particles in a solution based on light scattering, the fluorescence mode of the NTA instrument selectively detects only the fluorescently-labeled particles—in this case, the ExoGlow-NTA-labeled EVs (B)—and only the data from fluorescently-labeled particles is reported.

Supporting data

Get Better NTA data on EVs using ExoGlow-NTA

SBI’s ExoGlow-NTA Fluorescent Labeling Kit delivers highly accurate EV quantitation in a quick and easy workflow. The proprietary dye offers highly efficient labeling (Figure 2), very low background (Figure 3), and is compatible with a wide range of EV isolation techniques (Figure 4).

Figure 2. ExoGlow-NTA-labeled liposomes deliver consistent NTA data whether in light scattering or fluorescent mode. The high concordance of NTA and fluorescent NTA data collected from the ExoGlow-NTA Kit internal standards (ExoGlow-NTA-labeled synthetic liposomes) demonstrates the labeling efficiency of the ExoGlow-NTA Dye and accuracy of the fluorescent NTA method for characterizing EVs.

Figure 3.ExoGlow-NTA exhibits undetectable background signal. Conventional NTA and Fluorescent NTA of ExoGlow-NTA dye in the absence of EVs shows bias-free undetectable autofluorescence, based on (A) particle counts and (B) imaging.

Figure 4. ExoGlow-NTA demonstrates that conventional NTA overestimates EV concentration in samples irrespective of EV isolation method. Representative data comparing conventional NTA and fluorescent NTA for EVs isolated using (A) ExoQuick (10 μg serum protein), (B) ultracentrifugation and wash (1 μg serum protein), or (C) column based (1 μg serum protein), shows how much of the conventional NTA signal is due to non-EV particles.

More Exosome Services

Exo-NGS Service
Exosome Lipidomics/Metabolomics Service
Exosome Mass Spectrometry Service