EV-Luminite™ pCDH-MSCV-NLuc-CD81- EF1-Puro Expression Lentivirus

Product Description

EV-Luminite^TM is an innovative product line designed to revolutionize the field of Extracellular Vehicles (EVs) research and analysis. With its advanced technology and unique approach, EV-Luminite enables the precise quantification of subpopulation EV secretion and delivery by harnessing a luminescent signal. The cutting-edge products allow researchers and scientists to shed light on the dynamics and magnitude of EV release, transfer and subpopulation EV function, opening new avenues for understanding cellular communication and therapeutic applications, including studies on the biodistribution of EVs in vivo.

For the creation of EV-Luminite™ pCDH-MSCV-NLuc-CD81- EF1-Puro Expression Lentivirus, Nluc enzyme was cloned into an CD81 encoding lenti construct at the N-terminal position with puromycin resistance.

How the EV-Luminite^TM System Works

EV- Luminite works by utilizing Nano luciferase detection system to quantify the production/secretion of Extracellular Vesicles (EVs), evaluate EV delivery and even tracing the cellular communication of EV.

Target cells are transduced with EV-Luminite lentivirus to generate a EV-Luminite stable cell line from which EVs will be secreted.

EVs are isolated from the supernatant of the stable cell line and can be subsequently:

• qualified by nano luciferase assay
• delivered to recipient cells to assess EV delivery/uptaken
• delivered in mice to study EV dynamic biodistribution

The EV-Luminite stable cell line can be used for any customized high-content screening, such as screening for compound that can increase EV secretion, et al.

Supporting Data

Figure 2: EV derived from EV-Luminite Stable cell lines reveal strong Nano Luciferase activity. EV were isolated from Nluc-CD9 and Nluc-CD81 EV-Luminite Stable cell lines using ExoQuick-TC. Nano luciferase activity was measured using Nano-Glo Luciferase Assay and normalized by EV protein amount.

Figure 3: EV-Luminite serves as a useful tool to assess the effect of reagent on EV secretion/production. Nluc-CD9 and Nluc-CD81 EV-Luminite stable cells were treated with DMSO or Bafilomycin A1 (100nM) for over 5 hours. EV were isolated using ExQuick-TC. Nano luciferase activity of isolated EV was measured using Nano-Glo luciferase assay normalized by EV protein amount.