DriverMap™ EXP Human Genome-wide 19K Dried Blood Microsamples Profiling Kit, V3

Robust measurement of expression levels of 19,000 human protein-coding genes

Product Description

The DriverMap™ Targeted RNA Sequencing Expression Profiling (EXP) Assay is a convenient single-tube RT-PCR / Next-Generation sequencing (NGS) assay that robustly measures expression levels of 19,000 human protein-coding genes from ng levels of total RNA from blood, cells, tissue, even FFPE samples.  The DriverMap EXP kit includes all the enzymes, reagents, and primers to prepare NGS-ready libraries from total RNA. Open-source Salmon software is used to quantify expression levels for each gene. Further downstream analysis may be carried out using a variety of differential expression tools.

• This innovative solution for transcriptomic analysis of small volumes of whole blood (e.g. 30 μl) is a convenient and reliable way of obtaining high-throughput gene expression data for monitoring gene regulation and expression for biomarker and drug discovery applications. With this method, easy sample collection from remote locations without access to traditional blood collection facilities is possible.
• The DriverMap EXP Dried Blood kit contains additional reagents for RNA extraction from the Mitra tips as well as all the other primers and reagents required for NGS library prep included in the original DriverMap EXP Profiling kit.
• To use the DriverMap EXP Dried Blood protocol, you will need to order the Neoteryx Mitra tips and GenTegra GTR-NEO Active Chemical Protection of RNA solution separately from the Neoteryx site according to the table below:

How It Works

Fig.1: Outline of DriverMap EXP hGW19K assay for expression analysis of mRNA targets in dried blood microsamples

Performance Data

Fig.2: Reproducible and sensitive expression profiling with >90% overlap in gene expression profiles obtained from two different donors whose blood was extracted using standard blood collection tubes (400 ul) and Mitra tips (30 ul), with strong correlation (r=0.94) between results.