pPACK-SPIKE N501Y Combo Kit, includes Cat# CVD19-560A-1, plus PureFection Transfection Reagent and PEG-it Virus Concentration solution

Product Description

• Based on SBI’s popular and highly cited pPACKH1 Packaging System
• Uses codon-optimized SARS-CoV-2 N501Y "S" protein in place of VSV-G envelope protein
• Ideal for vaccine and antiviral efficacy studies under BSL2 conditions
• Package any 3rd-generation lentivector reporter of your choice, including SBI’s popular LentiLabeler reporters
• Use with pPACK-BALD™, an envelope protein-free lentivector packaging system that is an ideal negative control

Designed to efficiently package most third-generation lentivectors, the pPACK-SPIKE N501Y SARS-CoV-2 "S" Pseudotype – N501Y Mutant – Lentivector Packaging Mix speeds and simplifies preparation of lentiviral particles pseudotyped with the SARS-CoV-2 N501Y Spike glycoprotein. This variant is found in mouse-adapted SARS-CoV-2 and is used in mouse models of infection [1], making pPACK-SPIKE N501Y the ideal reagent for vaccine and antiviral drug discovery projects.

Based on SBI’s highly cited pPACKH1 packaging system but with a truncated SARS-CoV-2 N501Y Spike protein [2] replacing the standard VSV-G envelope protein, the pPACK-SPIKE N501Y Packaging Mix consists of three plasmids that produce all of the structural and replication proteins needed to transcribe and package an RNA copy of an expression lentivector into recombinant, N501Y "Spike" pseudotyped lentiviral particles. As a result, you can conduct a range of SARS-CoV-2 studies under BSL-2 conditions, including neutralization assays, studies of virus interactions with host surface proteins, and the development of vaccines and therapeutics.

For added convenience we also offer pPACK-BALD™, an envelope protein-free lentivector packaging mix that can be used as a negative control for pPACK-SPIKE N510Y studies, or for creating lentivirus particles pseudotyped with the envelope protein of your choice.

pPACK-SPIKE N501Y SARS-CoV-2 “S" Pseudotype -N501Y Mutant - Lentivector Packaging Mix is available as stand-alone packaging mixes with sufficient plasmids for 10 reactions (standard size) or 25 reactions (XL), as well as in a convenient Combo Kit that includes PureFection™ Transfection Reagent and PEG-it Virus Precipitation Solution.

How It Works

pPACK-SPIKE is easy to use - simply co-transfect the pPACK-SPIKE Plasmids and your lentivector construct into 293TN producer cells or other lentiviral production cells, isolate pseudoviral particles with an optional concentration step, and conduct your studies.