Lentivirus-based Gene Expression

Profit from a comprehensive range of well-established and innovative products

Leverage our tools from several top suppliers with exceptional experience in lentivirus technology. Take advantage of our wide selection of high-quality products to use lentiviral vectors as most effective vehicles for the delivery to and expression of a genetic element in almost any mammalian cell type - including non-dividing cells and model organisms.

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Stable, long-term expression of your target gene
In comparison to traditional retroviral delivery systems, lentivectors actively integrate into genomic DNA without requiring cell replication. They are the most effective vehicles for the delivery to and expression of a genetic element in almost any mammalian cell type - including non-dividing cells and model organisms.

As with standard plasmid vectors, it is possible to introduce lentivector expression constructs in plasmid form into the cells with low-to-medium efficiency using conventional transfection protocols. However, by packaging the lentivector construct into viral particles, you can obtain highly efficient transduction - even with the most difficult to transfect cells, such as primary, stem and differentiated cells.

The expression construct transduced in target cells is integrated into genomic DNA and provides stable, long-term expression of the target gene.

Tools for Lentiviral Gene Expression

Lentiviral Packaging Systems

To achieve highly efficient delivery and stable expression, the lentiviral constructs can be packaged into VSV-G pseudoviral particles using the respective

Cloning and Expression

Choose from an extended set of Lentivectors

Lentivirus Concentration and Titering

Lentivirus concentration & purification kits provide a fast method to concentrate your virus and purify with better results in less time than ultracentrifugation.

Concentrate and cryoprotect your lentiviral particles in one step with SBI’s top selling product PEG-it.

Quantitation of lentivirus is an important step to ensure efficient lentivirus expression. Choose between several sensitive assays to determine the physical virus titer.

Propagation of Lentiviral Constructs

Learn about Endura competent E. coli cells, the cells of choice for propagating lentiviral constructs, e.g. CRISPR Cas9 lentiviral gRNA constructs and libraries, such as the GeCKO (Genome Scale CRISPR Knock Out) pooled lentiviral gRNA libraries.

Custom Services

Save yourself the work of packaging lentiviral plasmids yourself. Obtain ready-to-transduce, pre-packaged, VSV-g pseudotyped lentiviral particles. We can provide any of Cellecta's lentiviral constructs, libraries, or other non-Cellecta lenti-constructs (on a case-by-case basis). The lentiviral particles fuse with the cell membranes and the lentiviral RNA enters the cell, is reverse-transcribed, and integrated into the cell’s DNA.

Lentivirus-based Tools for SARS-CoV-2 Research

Accelerate critical research and enhance understanding of the biology of SARS-CoV-2 variants with SARS-CoV-2 Spike Mutant-Pseudotyped Lentivirus Products. They comprise packaging kits as well as ready-to-use pseudoviral particles and enable assays in a biosafety level 2 laboratory to study virus-receptor interaction and to measure how antibodies or other compounds affect viral binding and entry.

Browse SARS-CoV-2 Spike Mutant-Pseudotyped Lentivirus Products

In addition we can also provide a Custom Lentivirus Pseudotyping Service. If you are interested in a Virus Receptor Cell Line Engineering Service please contact us.