E. cloni 10G (comparable to DH5alpha) SUPREME Electrocompetent Cells (SOLOs) > 4 x 10e10 cfu/ug

Product Description

Clone, construct libraries or transform Gibson Assembly® reactions all with high-yield, T1 phage-resistant cells in high efficiencies for routine and challenging applications.

• Direct replacements for standard cloning strains
• Optimised genetics for high yields:
  - Phage T1 resistant
  - Endonuclease and recombination minus
  - Blue/white screening-capable
• High efficiency cells result in high colony counts

Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (25ul aliquots for individual transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA.

E. cloni 10G SUPREME Electrocompetent Cells are also offered as DUOs.

Features

• Transformation Efficiency ≥ 4 × 10¹⁰ cfu/µg pUC DNA
• Ideal for Gibson Assembly

Genotype

F^- mcrA ∆(mrr-hsdRMS-mcrBC) endA1 recA1 φ80dlacZ∆M15 ∆lacX74 araD139 ∆(ara,leu)7697 galU galK rpsL (StrR) nupG λ− tonA

E. cloni 10G Competent Cells are available in 3 different efficiencies:

E. cloni 10G SUPREME Electrocompetent Cells ≥ 4 × 10¹⁰ cfu/µg pUC DNA

E. cloni 10G ELITE Electrocompetent Cells ≥ 2 × 10¹⁰ cfu/µg pUC DNA

E. cloni 10G CLASSIC Electrocompetent Cells ≥ 5 × 10⁹ cfu/µg pUC DNA