E. cloni 10G (comparable to DH5alpha) SUPREME Electrocompetent Cells (DUOs) > 4 x 10e10 cfu/ug
| Specifications | |
|---|---|
| Competence: | Electroporation Competent |
| Application: | Library Construction, General Cloning, Gibson Assembly |
| Organism: | E. coli |
Product Description
Clone, construct libraries or transform Gibson Assembly® reactions all with high-yield, T1 phage-resistant cells in high efficiencies for routine and challenging applications.
- Direct replacements for standard cloning strains
- Optimised genetics for high yields:
- Phage T1 resistant
- Endonuclease and recombination minus
- Blue/white screening-capable - High efficiency cells result in high colony counts
Competent Cells include Control DNA and Recovery Medium, and are packaged as DUOs ( (50ul aliquots for 2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA.
E. cloni 10G SUPREME Electrocompetent Cells are also offered as SOLOs.
Features
- Transformation Efficiency ≥ 4 × 1010 cfu/µg pUC DNA
- Ideal for Gibson Assembly
Genotype
F- mcrA ∆(mrr-hsdRMS-mcrBC) endA1 recA1 φ80dlacZ∆M15 ∆lacX74 araD139 ∆(ara,leu)7697 galU galK rpsL (StrR) nupG λ− tonA
E. cloni 10G Competent Cells are available in 3 different efficiencies:
E. cloni 10G SUPREME Electrocompetent Cells ≥ 4 × 1010 cfu/µg pUC DNA
E. cloni 10G ELITE Electrocompetent Cells ≥ 2 × 1010 cfu/µg pUC DNA
E. cloni 10G CLASSIC Electrocompetent Cells ≥ 5 × 109 cfu/µg pUC DNA
Custom Configurations
Please contact us for more information related to custom competent cell services or to receive a bulk quotation.
