RealSeq-Biofluids NGS Library Preparation Kit for miRNAs and small RNAs, for total RNA samples from biofluids (Illumina Platform), includes size selection reagent Add to Cart
RealSeq®-Biofluids is a kit for small RNA library preparation that is compatible with Illumina Next-generation sequencing (NGS) technology.
RealSeq®-Biofluids is optimized for cf-RNA from biofluid samples (50ul sample volume and up).
Libraries prepared with RealSeq®-biofluids yield substantially more miRNAs compared to competitor kits:
Sequencing results obtained from commercially available library preparation kits were compared using 200-ul aliquots from the same plasma samples. Short reads are classified as reads <15 nt length after adapter trimming. Reads passing this filter are then aligned to a reference file with all human miRNAs (miRBase 21, orange), and the remaining reads are aligned to the human genome (hg19, red).
RealSeq® - biofluids yields significantly fewer short or unmappable reads (grey) than the other two commercial libraries available that are indicated for biofluids analysis.
The number of sequenced miRNAs and of other mappable small RNAs is substantially higher.
RealSeq®-biofluids allows sequencing of significantly more miRNAs:
Comparison of miRNAs read frequencies of three different library preparation kits (gel-free protocols) from 200 ul plasma samples. Reads were subsampled to 10 million reads per kit and aligned to a reference that includes all human miRNAs listed in miRBase 21 (Miltenyi Biotec). The number of miRNAs detected at different coverage for each library preparation kit is shown.
Especially low copy-number miRNAs are significantly more sequenced when the RealSeq® - biofluids kit was used for library preparation.