Total RNA Clean-Up and Concentration Kit Add to Cart
Cat#: | 43200-NB |
Quantity: | 100 preps |
Price: |
542 €
Special 487.80 € - valid until 31 March 2019 |
Supplier: | Norgen |
Shipping: | RT |
For rapid and efficient clean-up and concentration of total RNA, including microRNA, without phenol
Please note: This kit can be used instead of discontinued #52900-NB
• Clean-up and concentrate total RNA isolated by different methods including phenol-chloroform extractions or alcohol precipitations
• Fast and easy processing - Rapid spin-column format allows for the processing of 10 samples in 20 minutes, while the 96-well plates can be processed in 30 minutes
• No phenol or chloroform extractions
• Clean-up RNA from contaminants including enzymes, RNases and nucleotides
• Isolate all small RNA molecules including microRNA, siRNA, tRNA, and 5S rRNA
• Purified RNA is fully compatible with all downstream applications
• Also available in a 96-well format for high throughput applications
Norgen’s RNA Clean-Up and Concentration Micro Kit provides a rapid method for the purification, cleanup and concentration of up to 35 µg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other reaction components such as proteins, RNases and nucleotides, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including end-point or quantitative reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Workflow
Effective Clean-Up to Produce High Quality Total RNA with Complete Size Diversity. Norgen’s RNA Clean-Up and Concentration Kit effectively cleans up RNA isolated from phenol-based extractions without the loss of RNA diversity by retaining all RNA species including small RNAs. Total RNA was isolated from 5 x 108 E. coli using a competitor’s phenol-based RNA extraction reagent. The resulting RNA was then purified using Norgen’s RNA Clean-Up and Concentration Kit. As controls, total RNA was extracted using both Norgen´s Total RNA Purfication Kit (#17200, no phenol required) and the phenol-based RNA extraction reagent only without any clean-up. Resolution of 7 µL of the 50 µL purified RNA on a 1X MOPS, 1.5% formaldehyde-agarose gel showed the RNA extracted with phenol was successfully cleaned-up by Norgen’s RNA Clean-Up and Concentration Kit without the loss of small RNA species.
Effective Clean-Up to Produce High Quality Total RNA Compatible to Bioanalyzer Analysis. Total RNA was isolated from 0.75 million HeLa cells using a competitor’s phenol-based RNA extraction reagent. The resulting RNA was then purified using Norgen’s RNA Clean-Up and Concentration Kit. As controls, total RNA was extracted using both Norgen´s Total RNA Purfication Kit (#17200, no phenol required) or the phenol-based RNA extraction reagent only without clean-up. Resolution of 1 µL of the 50 µL purified RNA on an Agilent RNA Nano 6000 Chip showed that without clean-up, the RNA sample isolated using the phenol-based RNA extraction reagent only resolved poorly on a bioanalyzer. In contrast, RNA extracted with the phenol-based RNA extraction reagent followed by clean-up by Norgen’s RNA Clean-Up and Concentration Kit resolved properly on the bioanalyzer showing excellent quality.
Effective Concentration and Detection of Low Amounts of RNA Input. Norgen’s RNA Clean-Up and Concentration Kit can effectively concentration RNA inputs in the picogram range. Increasing amounts of HeLa RNA in 50 µL input volumes were concentrated to 20 µL using Norgen’s RNA Clean-Up and Concentration Kit. A 10 µL aliquot of the purified RNA was then used as the template in a qRT-PCR reaction to detect the S14 gene. The amounts indicated on the graph correspond to the amount of RNA that was used as the input for the qRT-PCR reaction, demonstrating the consistent performance of the kit even in the picogram range.
- Al-Sheikh YA, Ghneim HK, Softa KI, Al-Jobran AA, Al-Obeed O, Mohamed MA, Abdulla M, Aboul-Soud MA. (2016) Expression profiling of selected microRNA signatures in plasma and tissues of Saudi colorectal cancer patients by qPCR. Oncology Letters
- Seifer, B. J., Su, D., & Taylor, H. S. (2016) Circulating miRNAs in Murine Experimental Endometriosis Decreased Abundance of let-7a. Reproductive Sciences
- Brian S. Roberts, Andrew A. Hardigan, Marie K. Kirby, Meredith B. Fitz-Gerald, C. Mel Wilcox, Robert P. Kimberly and Richard M. Myers. (2015) Blocking of targeted microRNAs from next-generation sequencing libraries. Nucleic Acids Research
- David Preskey, Thomas F. Allison, Mark Jones, Kamel Mamchaou, and Christian Unger. (2015) Synthetically modified mRNA for efficient and fast human iPS cell generation and direct transdifferentiation to myoblasts. Biochemical and Biophysical Research Communications
- Alessandra Drusco, Arianna Bottoni, Alessandro Lagana, Mario Acunzo, Matteo Fassan, Luciano Cascione, Anna Antenucci, Prasanthi Kumchala, Caterina Vicentini, Marina P Gardinam, Hansjuerg Alder, Mariantonia A Carosi, Mario Ammirati, Stefano Gherardi, Marilena Luscri, Carmine Carapella, Nicola Zanesi, Carlo M Croce. (2015) A differentially expressed set of micrornas in cerebro-spinal fluid (CSF) can diagnose CNS malignancies. Oncotarget
- Fagerlind M, Stålhammar H, Olsson B, Klinga-Levan K. (2015) Expression of miRNAs in Bull Spermatozoa Correlates with Fertility Rates. Reproduction in Domestic Animals
- Tatiana Rochat, Olivier Delumeau, Nara Figueroa-Bossi, Philippe Noirot, Lionello Bossi, Etienne Dervyn, Philippe Bouloc. (2015) Tracking the Elusive Function of Bacillus subtilis Hfq. PLOS ONE
- R.K. Chilukoti, A. Giese, W. Malenke, G. Homuth, A. Bukowska, A., Goette, S.B. Felix, J. Kanaan, H.-G. Wollert, K. Evert, S. Verheule, P. Jais, S.N. Hatem, U. Lendeckel, C. Wolke. (2015) Atrial fibrillation and rapid acute pacing regulate adipocyte/adipositas-related gene expression in the atria. International Journal of Cardiology
- Erik Richter, Manuela Harms, Katharina Ventz, Philipp Gierok, Ravi Kumar Chilukoti, Jan-Peter Hildebrandt, Jörg Mostertz, Falko Hochgräfe. (2015) A Multi-Omics Approach Identifies Key Hubs Associated with Cell Type-Specific Responses of Airway Epithelial Cells to Staphylococcal Alpha-Toxin. PLOS ONE
- Andriy Bilichak, Yaroslav Ilnytskyy, Rafal Wóycicki, Nina Kepeshchuk, Dawson Fogen and Igor Kovalchuk. (2015) The elucidation of stress memory inheritance in Brassica rapa plants. Frontiers in Plant Science
- Sashi Kant, Shivani Agarwal, Preeti Pancholi and Vijay Pancholi. (2015) The Streptococcus pyogenes Orphan Protein Tyrosine Phosphatase, SP-PTP, Possesses Dual Specificity, and Essential Virulence Regulatory Functions. Molecular Microbiology
- M Mura, T G Hopkins, T Michael, N Abd-Latip, J Weir, E Aboagye, F Mauri, C Jameson, J Sturge, H Gabra, M Bushell, A E Willis, E Curry and S P Blagden. (2014) LARP1 post-transcriptionally regulates mTOR and contributes to cancer progression. Oncogene
- Sarah C. Ferrante, Evan P. Nadler, Dinesh K. Pillai, Monica J. Hubal, Zuyi Wang, Justin M. Wang, Heather Gordish-Dressman, Emily Koeck, Samantha Sevilla, Andrew A. Wiles and Robert J. Freishtat. (2014) Adipocyte-derived exosomal miRNAs: a novel mechanism for obesity-related disease. Pediatric Research
- Jessica Mühlhaus, Juliane Dinter, Daniela Nürnberg, Maren Rehders, Maren Depke, Janine Golchert, Georg Homuth, Chun-Xia Yi, Silke Morin, Josef Köhrle, Klaudia Brix, Matthias Tschöp, Gunnar Kleinau and Heike Biebermann. (2014) Analysis of Human TAAR8 and Murine Taar8b Mediated Signaling Pathways and Expression Profile. International Journal of Molecular Sciences
- Berthold T, Muschter S, Schubert N, Wesche J, Ameling S, Teumer A, Reil A, Bux J, Bakchoul T, Greinacher A. (2014) Impact of priming on the response of neutrophils to human neutrophil alloantigen-3a antibodies. Transfusion
- A. Koneti Rao, Robert J. Freishtat, Gauthami Jalagadugula, Anamika Singh, Guangfen Mao, Andrew Wiles, Peter Cheung, Guenther Boden. (2014) Alterations in Insulin-Signaling and Coagulation Pathways in Platelets during Hyperglycemia-Hyperinsulinemia in Healthy Non-Diabetic Subject. JThrombosis Research
- Shulu Zhang, Michel Ravelonandro, Paul Russell, Nathan McOwen, Pascal Briard, Seven Bohannon, Albert Vrient. (2014) Rapid diagnostic detection of plum pox virus in Prunus plants by isothermal AmplifyRP® using reverse transcription-recombinase polymerase amplification. Journal of Virology Methods
- Decker C, Parker R. (2014) Analysis of Double-Stranded RNA from Microbial Communities Identifies Double-Stranded RNA Virus-like Elements. Cell Reports
- Handtke S, Schroeter R, Jürgen B, Methling K, Schlüter R, Albrecht D, Van Hijum S, Bongaerts J, Maurer K, Lalk M, Schweder T, Hecker M, Voigt B. (2014) Bacillus pumilus Reveals a Remarkably High Resistance to Hydrogen Peroxide Provoked Oxidative Stress. PLOS One
- Kabisch J, Pratzka I, Meyer H, Albrecht D, Lalk M, Ehrenreich A, Schweder T. (2013) Metabolic engineering of Bacillus subtilis for growth on overflow metabolites. Microbial Cell Factories
- Toffano-Nioche C, Ott A, Crozat E, Nguyen AN, Zytnicki M, Leclerc F, Forterre P, Bouloc P, Gautheret D. (2013) RNA at 92 °C: the non-coding transcriptome of the hyperthermophilic archaeon Pyrococcus abyssi. RNA Biology
- Iovino F, Orihuela CJ, Moorlag HE, Molema G, Bijlsma JJ. (2013) Interactions between Blood-Borne Streptococcus pneumoniae and the Blood-Brain Barrier Preceding Meningitis. PLoS One
- Chilukoti RK, Mostertz J, Bukowska A, Aderkast C, Felix SB, Busch M, Völker U, Goette A, Wolke C, Homuth G, Lendeckel U. (2013) Effects of irbesartan on gene expression revealed by transcriptome analysis of left atrial tissue in a porcine model of acute rapid pacing in vivo. International Journal of Cardiology
- Harmon B, Kozina C, Maar D, Carpenter TS, Branda CS, Negrete OA, Carson BD. (2013) Identification of Critical Amino Acids within the Nucleoprotein of Tacaribe Virus Important for Anti-Interferon Activity. The Journal of Biological Chemistry
- Pförtnera H, Buriana M, Michalik S, Depke M, Hildebrandt P, Dhople V, Pané-Farré J, Hecker M, Schmidt F, Völker U. (2013) Activation of the alternative sigma factor SigB of Staphylococcus aureus following internalization by epithelial cells - An in vivo proteomics perspective. International Journal of Medical Microbiology
- Löbler M, Buß D, Kastner C, Mostertz J, Homuth G, Ernst M, Guthoff R, Wree A, Stahnke T, Fuellen G, Voelker U, Schmitz P. (2013) Ocular fibroblast types differ in their mRNA profiles—implications for fibrosis prevention after aqueous shunt implantation. Comparative Biochemistry and Physiology
- Carlsen AL, Schetter AJ, Nielsen CT, Lood C, Knudsen S, Voss A, Harris CC, Hellmark T, Segelmark M, Jacobsen S, Bengtsson AA, Heegaard NH. (2013) Circulating microRNA expression profiles associated with systemic lupus erythematosus. Arthritis and Rheumatism
- Haga-Friedman A, Horovitz-Fried M, J Cohen C. (2012) Incorporation of Transmembrane Hydrophobic Mutations in the TCR Enhance Its Surface Expression and T Cell Functional Avidity. The Journal of Immunology
- Kabgani N, Grigoleit T, Schulte K, Sechi A, Sauer-Lehnen S, Tag C, Boor P, Kuppe C, Warsow G, Schordan S, Mostertz J, Chilukoti RK, Homuth G, Endlich N, Tacke F, Weiskirchen R, Fuellen G, Endlich K, Floege J, Smeets B, Moeller MJ. (2012) Primary cultures of glomerular parietal epithelial cells or podocytes with proven origin. PLoS One
- Elsholz AK, Turgay K, Michalik S, Hessling B, Gronau K, Oertel D, Mäder U, Bernhardt J, Becher D, Hecker M, Gerth U. (2012) Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis. Proceedings of the National Academy of Sciences of the United States of America
- Welte T, Kudva R, Kuhn P, Sturm L, Braig D, Müller M, Warscheid B, Drepper F, Koch HG. (2012) Promiscuous targeting of polytopic membrane proteins to SecYEG or YidC by the Escherichia coli signal recognition particle. Molecular Biology of the Cell
- Bukowska A, Hammwöhner M, Sixdorf A, Schild L, Wiswedel I, Röhl FW, Wolke C, Lendeckel U, Aderkast C, Bochmann S, Chilukoti RK, Mostertz J, Bramlage P, Goette A. (2012) Dronedarone prevents microcirculatory abnormalities in the left ventricle during atrial tachypacing in pigs. British Journal of Pharmacology
- Zhang D, Liu B, Zhao C, Lu X, Wan D, Ma F, Chen L, Liu J. (2012) Ecological functions and differentially expressed transcripts of translucent bracts in an alpine 'glasshouse' plant Rheum nobile (Polygonaceae). Planta
- Zweers JC, Nicolas P, Wiegert T, van Dijl JM, Denham EL. (2012) Definition of the σW Regulon of Bacillus subtilis in the Absence of Stress. PLOS One
- Ryan MM, Ryan B, Kyrke-Smith M, Logan B, Tate WP, Abraham W, Williams J. (2012) Temporal Profiling of Gene Networks Associated with the Late Phase of Long-Term Potentiation In Vivo. PLOS ONE
- Depke M, Burian M, Schäfer T, Bröker BM, Ohlsen K, Völker U. (2011) The alternative sigma factor B modulates virulence gene expression in a murine Staphylococcus aureus infection model but does not influence kidney gene expression pattern of the host. International Journal of Medicine Microbiology
- Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J. (2011) RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. Molecular Microbiology
- Chi BK, Gronau K, Maeder U, Hessling B, Becher D, Antelmann H. (2011) S-bacillithiolation protects against hypochlorite stress in Bacillus subtilis as revealed by transcriptomics and redox proteomics. Molecular and Cellular Proteomics
- Zuber P, Chauhan S, Pilaka P, Nakano M, Gurumoorthy S, Lin A, Barendt S, Chi B, Antelmann H and Mäder U. (2011) Phenotype Enhancement Screen of a Regulatory spx Mutant Unveils a Role for the ytpQ Gene in the Control of Iron Homeostasis. PLOS One
- Falord M, Mader U, Hiron A, Dbarbouille M, Msadek T. (2011) Investigation of the Staphylococcus aureus GraSR Regulon Reveals Novel Links to Virulence, Stress Response and Cell Wall Signal Transduction Pathway. PLOS One
- Winter T, Winter J, Polak M, Kusch K, Mäder U, Sietmann R, Ehlbeck J, Hijum S, Weltmann K, Hecker M, Kusch H. (2011) Characterization of the global impact of low temperature gas plasma on vegetative microorganisms. Proteomics
- K. Saveetha, L. Karthiba, M. Raveendran, D. Saravanakumar, T. Raguchander and R. Samiyappan. (2010) Transcriptional analysis of molecular interactions between Pseudomonas fluorescens strain TDK1, Oryza sativa and Cnaphalocrocis medinalis. Journal of Applied Entomology
- M. Dassanayake, J. S. Haas, H. J. Bohnert and J. M. Cheeseman. (2009) Shedding light on an extremophile lifestyle through transcriptomics. New Phytologist
- Gioia J, Yerrapragada S, Qin X, Jiang H, Igboeli OC, Muzny D, Dugan-Rocha S, Ding Y, Hawes A, Liu W, Perez L, Kovar C, Dinh H, Lee S, Nazareth L, Blyth P, Holder M, Buhay C, Tirumalai MR, Liu Y, Dasgupta I, Bokhetache L, Fujita M, Karouia F, Eswara Moorthy P, Siefert J, Uzman A, Buzumbo P, Verma A, Zwiya H, McWilliams BD, Olowu A, Clinkenbeard KD, Newcombe D, Golebiewski L, Petrosino JF, Nicholson WL, Fox GE, Venkateswaran K, Highlander SK, Weinstock GM. (2007) Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032. PLoS ONE