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Total RNA Clean-Up and Concentration Kit Add to Cart

Cat#: 23600-NB
Quantity: 50 preps
Price: 303 €
Supplier: Norgen
Shipping: RT
User Manual  

Please note: This kit can be used instead of discontinued #52900-NB

Efficient clean-up from various enzymatic reactions - RNA clean-up from upstream enzymatic applications such as labeling, DNase treatment and in vitro transcription. Remove proteins, RNases, DNases, nucleotides, etc.

Clean-up and concentration of RNA isolated by different methods - RNA that has been isolated using various methods, including phenol-chloroform extractions or alcohol precipitations, can be processed with this kit

Fast and easy processing - Rapid spin-column format allows for the processing of 10 samples in 20 minutes

No phenol:chloroform extractions - The RNA Clean-Up and Concentration Kit purifies RNA without the use of harmful chemicals such as phenol or chloroform

Isolate all small RNA molecules - All small RNA species can be isolated including microRNA, siRNA, tRNA, and 5S rRNA

Recovered RNA is suitable for downstream applications - Purified RNA can be used in a number of downstream applications including end-point or quantitative reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array analysis


Kit Specifications

Column binding capacity: 50 µg
Maximum amount of starting material: 50 µg of total RNA
Minimum elution volume: 20 µl
Size of RNA purified: all sizes
Average recovery: >90%
Time to complete 10 purifications: 20 minutes


Applications

• Quantitative, real-time RT-PCR
• RT-PCR
• Northern blotting
• RNase protection and primer extension
• Expression array assays



Image
High quality RNA.Total RNA was isolated from HeLa cells using TRI Reagent, which relies on organic extractions. The resulting RNA was then purified using Norgen’s RNA Clean-Up and Concentration Kit, and a portion of the purified RNA was then run on an Agilent 2100 Bioanalyzer to demonstrate the high quality of the purified RNA.



Image
Concentration and detection of increasing amounts of HeLa RNA..Increasing amounts of HeLa RNA in 50 µl input volumes were concentrated to 20 µl using Norgen’s RNA Clean-Up and Concentration Kit. A 10 µl aliquot of the purified RNA was then used as the template in a qRT-PCR reaction to detect the S14 gene. The amounts indicated on the graph correspond to the amount of RNA that was used as the input for the qRT-PCR reaction, demonstrating the consistent performance of the kit even in the picogram range.

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Effect of microRNA GC Content on RNA Purification Efficiency


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