MosaiX™ DNA Library Prep Kit
Complexity Made Simple
Product Description
Combine the workflow simplicity of tagmentation, the reduced insertion bias of TnX, and the performance benefits of ligation.
- With engineered TnX transposase for uniformity of coverage
- Up to 50% faster than alternative technologies
- Simple 90-minute workflow (35 minutes or less hands-on)
- Reduced labor and increased efficiency
- Produces high complexity libraries
- Low duplication rates
The MosaiX Library prep kit employs a proprietary chemistry based on directional tagmentation and incorporates TnX, an engineered transposase. TnX was developed through directed enzyme evolution for reduced insertion bias, improved activity, and enhanced inhibitor tolerance. Within the MosaiX workflow, DNA is fragmented and Read 1 adapters added to the 5’ end via TnX followed by 3' ligation of Read 2 adapters, generating a high proportion of productive library molecules. The streamlined workflow produces highquality ready-to-sequence libraries in just 90 minutes and is ideal for applications like human whole genome and whole exome sequencing as well as targeted hybridization capture.
50% Faster Generation of Highly Complex Libraries
seqWell Directional Tagmentation
MosaiX 90-minute Workflow
Kit Content
All enzymes, buffers, adapters, and beads required to generate 24 or 96 libraries are included:
- TnX Read 1 Tagging Reagent
- 5X Reaction Buffer
- Ligation Enhancer
- Read 2 Adapter
- DNA Ligase
- 2X Amplification Ready Mix
- MAGwise Paramagnetic Beads
- Diluent
Specifications
* < 5 ng may require optimization of adapter concentration and PCR cycles
** Fragment size optimized for NovaSeq X Plus
Performance Data
Whole Exome Sequencing
Exome metrics at 6 Gb for libraries generated using MosaiX Library Prep were extremely comparable to libraries generated using enzymatic fragmentation followed by ligation. Additionally, MosaiX Library Prep outperformed bead-linked Tn5 transposition in terms of duplication rate, library complexity (HS Library Size), and % of Zero Coverage Targets – highlighting the TnX performance difference!
Method: 50 ng of NA12878 DNA (Genome in a Bottle) was used for all and libraries were prepared following manufacturers’ user guides. Libraries were then captured using Twist’s Exome 2.0 panel and standard workflow. Sequencing was performed on NextSeq 2000, down-sampled to 6 Gb each, then aligned to Twist exome capture targets on hg38.
TnX finds those missing exome targets!
- Clinically relevant targets can be missed when using other library preparation methods such as bead-linked tagmentation using Tn5
- Reduced TnX insertion bias and higher molecular complexity of MosaiX libraries can access difficult regions of the genome that Tn5 transposase cannot.
IGV plot showing clinically relevant targets present in MosaiX that are missing in libraries prepared using bead-linked Tn5.
Whole Genome Sequencing
MosaiX libraries achieve higher coverage compared to libraries prepared with bead-linked Tn5 transposition with the same total PF Gb. Duplication rate is lower and estimated library size is higher for MosaiX, indicating a more complex whole genome library.
Method: 50 ng of NA12878 DNA (Genome in a Bottle) was used in both and libraries were prepared following manufacturers’ user guides. Sequencing was performed on a lane of a NovaSeq X+ 25B flow cell, down-sampled to 105 Gb each, then aligned to hg38.
Additional Resources
MosaiX Index List (download)
MosaiX: High-Performance DNA Library Prep with Directional Tagmentation Powered by TnX (Poster)
Enabling Community-Driven Genetics of the Domestic Cat at Large Scale (Poster)
