A gene circuit executed in any myTXTL® kit is required to start with a σ70-specific promoter, as gene expression in myTXTL® relies entirely on the endogenous TXTL machinery of E. coli. Its modular and pre-designed format makes it also ideal for educational purposes to train students and demonstrate basic concepts of synthetic biology.
Visit our Cell-free Protein Expression page to learn more about the myTXTL System.
Please note: The plasmid sample you receive upon ordering is intended for propagation purposes only. To make the plasmid suitable for in vitro expression in myTXTL, follow the recommended 2-step procedure to generate high-purity plasmid samples. For propagation of P70a-vector, please also read the Tech Note about “Preparation of chemo-competent KL740 cells for amplification of P70a vectors“.
Two plasmids are available in high purity, ready-to-use versions: