The PiggyBac (PB) transposon is a mobile genetic element that efficiently transposes between vectors and chromosomes via a "cut and paste" mechanism. During transposition, the PB transposase recognizes transposon-specific inverted terminal repeat sequences (ITRs) located on both ends of the transposon vector and efficiently moves the contents from the original sites and efficiently integrates them into TTAA chromosomal sites. The powerful activity of the PiggyBac Transposon System* enables genes of interest between the two ITRs in the PB vector to be easily mobilized into target genomes.
The unique features of PiggyBac transposons are that there is no cargo limit and it is also reversible. Genomes containing an inserted PiggyBac vector can be transiently re-transfected with the PB tranposase expression vector. The PB transposase will remove the transposons from the genome, footprint-free.
Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas Commercial customers must sign a customer agreement for a four-month, limited-use license to evaluate the technology.
For end user license information, see the following:
* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Hera BioLabs, Inc.