Recombinant Human Noggin/NOG Protein (His Tag)(Active)
| Specifications | |
|---|---|
| Molecular Weight: | 24.6 kDa |
| Tag: | C-His |
| Source: | HEK293 Cells |
| Purity: | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin Level: | < 1.0 EU per µg as determined by the LAL method. |
| Activity: | Measured by its ability to inhibit recombinant human BMP4-induced alkaline phosphatase production by MC3T3-E1 cells.The ED50 for this effect is typically 0.05-0.3 μg/mL in the presence of 50 ng/mL of recombinant human BMP4. |
| Format: | Lyophilized from sterile PBS, pH 7.4 |
| Additional Information | |
|---|---|
| Synonyms: | Noggin;SYM1;SYNS1 |
| Organism: | Human |
| Accn. No.: | NP_005441.1 |
Sequence Information
Met 1-Cys 232
Background
Noggin is a secreted protein involved at multiple stages of vertebrate embryonic development including neural induction and is known to exert its effects by inhibiting the bone morphogenetic protein (BMP)-signaling pathway. It binds several BMPs with very high (picomolar) affinities; with a marked preference for BMP2 and BMP4 over BMP7. By binding tightly to BMPs; Noggin prevents BMPs from binding their receptors. Noggin binds the bone morphogenetic proteins (BMP) such as BMP-4 and BMP-7; and inhibits BMP signaling by blocking the molecular interfaces of the binding epitopes for both type I and type II receptors. Interaction of BMP and its antagonist Noggin governs various developmental and cellular processes; including embryonic dorsal-ventral axis; induction of neural tissue; formation of joints in the skeletal system and neurogenesis in the adult brain. Noggin plays a key role in neural induction by inhibiting BMP4; along with other TGF-β signaling inhibitors such as chordin and follistatin. Mouse knockout experiments have demonstrated that noggin also plays a crucial role in bone development; joint formation; and neural tube fusion.
Price
609,00 €
