Recombinant Human Noggin/NOG Protein (His Tag)(Active)

Specifications
Molecular Weight:24.6 kDa
Tag:C-His
Source:HEK293 Cells
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin Level:< 1.0 EU per µg as determined by the LAL method.
Activity:Measured by its ability to inhibit recombinant human BMP4-induced alkaline phosphatase production by MC3T3-E1 cells.The ED50 for this effect is typically 0.05-0.3 μg/mL in the presence of 50 ng/mL of recombinant human BMP4.
Format:Lyophilized from sterile PBS, pH 7.4
Additional Information
Synonyms:Noggin;SYM1;SYNS1
Organism:Human
Accn. No.:NP_005441.1

Sequence Information

Met 1-Cys 232

Background

Noggin is a secreted protein involved at multiple stages of vertebrate embryonic development including neural induction and is known to exert its effects by inhibiting the bone morphogenetic protein (BMP)-signaling pathway. It binds several BMPs with very high (picomolar) affinities; with a marked preference for BMP2 and BMP4 over BMP7. By binding tightly to BMPs; Noggin prevents BMPs from binding their receptors. Noggin binds the bone morphogenetic proteins (BMP) such as BMP-4 and BMP-7; and inhibits BMP signaling by blocking the molecular interfaces of the binding epitopes for both type I and type II receptors. Interaction of BMP and its antagonist Noggin governs various developmental and cellular processes; including embryonic dorsal-ventral axis; induction of neural tissue; formation of joints in the skeletal system and neurogenesis in the adult brain. Noggin plays a key role in neural induction by inhibiting BMP4; along with other TGF-β signaling inhibitors such as chordin and follistatin. Mouse knockout experiments have demonstrated that noggin also plays a crucial role in bone development; joint formation; and neural tube fusion.
  • Catalog Number
    PKSH031720-1mg-ELS
  • Supplier
    Elabscience
  • Size
  • Shipping
    RT
Price
3.813,00 €
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