RapiDxFire™ Lyo-Flex 1-step RT-qPCR 5X Master Mix
Designed for one-step viral/pathogen detection for both centralised and point of care (POC) workflows
Product Description
RapiDxFire™ Lyo-Flex 1-Step RT-qPCR 5X Master Mix is a convenient, single-tube master mix that provides sensitive and reproducible detection of bacterial and viral pathogens. Optimized for multiplexing with gene-specific primers and BHQ probes, this temperature-stable, glycerol-free, mix is ideal for molecular diagnostic kit manufacturers developing traditional tests for high-throughput, automated workstreams or lyophilised tests for POC devices.
- High sensitivity to detect low copy RNA and DNA targets in single- and multiplex reactions
- Single tube, room temperature-stable formulation streamlines automated workflows
- Glycerol-free components for flexible lyophilisation options
- 5X concentration allows increased sample addition in liquid reactions or smaller lyobead formation during manufacturing
Provided at a 5X concentration without passive reference dye, this robust master mix includes a heat-activated thermostable reverse transcriptase, Taq DNA polymerase, and dNTPs (including dUTP) in a single, Triton-free, glycerol-free buffered solution. Compatible with added Uracil N-Glycolsylase (UNG) to control carryover contamination and/or an RNase inhibitor for template preservation, RapiDxFire Lyo-Flex 1-Step RT-qPCR 5X Master Mix is a versatile component for diagnostic test development. RapiDxFire Lyo-Flex 1-Step RT-qPCR 5X Master Mix is manufactured in an ISO 13485-certified facility and is available in suitable test development sizes to large scale production lots.
For Research Use Only!
Supporting Data
Fig.1. Broad dynamic range for both RNA and DNA targets. (a) SARS-CoV-2 RNA control (Twist) 10-fold serially diluted from 1x106-1x100 copies per reaction, on which the UltraDx SARS-CoV-2 N1/N2/RnP assay (Biosearch Technologies) was performed (N1/N2:FAM shown) (20 µL total reaction volume). (b) genomic mouse DNA 10-fold serially diluted from 300,000 to 3 copies per reaction on which the Isg20 assay was performed (N1/N2:FAM shown) (20 µL total reaction volume). PCR efficiencies (90%-110%) and R2 values >0.98 for (a) SARS-CoV-2 RNA and (b) genomic mouse DNA assays met PCR criteria.
