Plasma/Serum cfc-RNA Advanced Purification Kit

Product Description

• Isolate all sizes of circulating and exosomal RNA, including microRNA
• Versatile plasma/serum input ranging from 1 mL to 6 mL
• No phenol extractions
• No carrier RNA
• Bind and elute all RNA irrespective of size or GC content, without bias
• Concentrate circulating RNA and exosomal RNA into a flexible elution volume ranging from 25 µL to 50 µL
• Purify superior-quality and superior-quantity RNA in 45 minutes
• Fully automated purification procedure on Hamilton MicroLab Nimbus
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing

Norgen’s Plasma/Serum cfc-RNA Advanced Purification Kit provides a fast, reliable and simple procedure for isolating the highest quality and quantity of all sizes of circulating RNA, including microRNA, as well as all sizes of exosomal RNA from various amounts of plasma/serum ranging from 1 mL up to 6 mL with minimal DNA contamination. Purification is based on using Norgen’s proprietary resin separation matrix. The kit is designed to isolate all sizes of cfc-RNA from either fresh, preserved or frozen plasma/serum samples. Moreover, this kit allows the user to elute the purified cfc-RNA into a flexible elution volume ranging from 25 µL to 50 µL.

The purified plasma/serum cfc-RNA is eluted in an Elution Solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS. 

Performance Data

Figure 1. A representative Bioanalyzer RNA Pico Chip trace showing the quality/quantity of the RNA purified from 4 mL K2 EDTA plasma using A) Norgen’s Plasma/Serum cfc-RNA Advanced Purification Kit (Automated Isolation), B) Norgen’s Plasma/Serum cfc-RNA Advanced Purification Kit (Manual Isolation), C) Competitor Q's cfc-DNA/RNA Kit and D) Competitor Q's Circulating Nucleic Acid Kit. All purification methods showed the correct cf-RNA size on the bioanalyzer trace however the amount of the cf-RNA recovered using Norgen’s technology (as can be noticed by the height of the cf-RNA peak) was significantly higher than that for the cf-RNA purified using the silica-based technology.