Opti-Protein XL Marker

Product Description

Opti-Protein XL Marker/Ladder consists of 12 proteins that resolve into sharp, tight bands in the range of 10-245 kDa. This unique protein ladder allows you to monitor protein separation during electrophoresis, estimate molecular weight of the protein of interest, and evaluate western blot transfer efficiency. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).

Specifications

• Number of Bands: 12
• Protein Range: 10kDa-245kDa
• Suggested Load: Mix gently by pipetting up and down a few time, then directly load 5 μl into well of gel
  
  

Application

• Monitoring protein migration during SDS-PAGE
• Monitoring efficiency of protein transfer onto PVDF or nitrocellulose for Western Blotting
• Sizing of proteins on SDS-PAGE and Western Blots