HRP (Horseradish Peroxidase) Conjugated Affinity Purified anti-Goat IgG [H and L] [Rabbit]
| Specifications | |
|---|---|
| Type: | Secondary Antibody |
| Host: | Rabbit |
| Reactivity: | Goat |
| Label: | Horseradish Peroxidase (HRP) |
| Concentration: | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Buffer: | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Stabilizer: | 10 mg/ml BSA, IgG and Protease free |
| Preservative: | 0.01% (w/v) Gentamicin Sulfate |
Applications
Immunoblotting, ELISA, ELMI, IHC
Recommended Dilution
This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:14,000 to 1:60,000 of the reconstitution concentration is suggested for this product. Optimal titers for applications should be determined by the researcher.
Immunogen
Goat IgG whole molecule
Purification/Format
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against Human Serum Proteins.
Background
Minimum Cross reactivity to Human Serum Proteins
Additional Information
Minimum Cross reactivity to Human Serum Proteins
- Catalog Number
SH012-ABM - Supplier
ABM - Size
- Shipping
Blue Ice
Price
189,00 €
