FBS Exosome Depletion Kit I - Slurry Format (Deplete up to 140 ml FBS)

• Efficient depletion of cow's exosomes from Fetal Bovine Serum
• Deplete exosome-sized vesicles from versatile FBS volumes of up to 280 mL
• No protease treatment required
• No time-consuming ultracentrifugation
• No precipitation reagents required
• No overnight incubation required
• Depleted FBS has no detectable cow’s miRNA
• The depleted FBS provides the same cellular growth rates as the standard FBS
• Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon Carbide resin separation matrix

Norgen's FBS Exosome Depletion Kits (Slurry Format) constitute an all-in-one system for the depletion of cow's exosomes from FBS prior to using it as a growth supplement in your culture medium. The FBS recovered from the depletion process is exosome-depleted and does not contain any quantifiable bovine miRNAs. Moreover, the exosome-depleted FBS will support the growth of your cells of interest similar to the non-depleted FBS. Norgen's kits allows for the depletion of different FBS volumes with a maximum volume ranging from 140 mL to 280 mL. The depletion is based on Norgen’s proprietary resin. These kits provide a clear advantage over other available kits in that they do not require ultracentrifugation, any special instrumentation, precipitation reagents or any protease treatments. More importantly, the depletion process is an inexpensive method for depletion of your own FBS, as compared to the current ready-to-use exosome-depleted media available on the market.

Background

Most culture medium used for the growth and propagation of cells in culture require the addition of fetal bovine serum (FBS) as a growth complement to media. FBS is obtained from bovine (cow) serum, and therefore contains large quantities of cow exosome vesicles. These exosomes may interfere with some types of studies, or may lead to unreliable results when studying the exosomes shed from your cells of interest in normal culture conditions. Therefore, the use of exosome-depleted FBS is highly recommended for many types of studies.

Performance Data

Figure 1. Exosome-depleted FBS with Norgen's FBS Exosome Depletion Kits (Slurry Format) has undetectable Bovine miRNA levels. Norgen's FBS Exosome Depletion Kit I (Slurry Format) (Cat# 61100) was used to deplete bovine miRNA from 5mL FBS. Total RNA/miRNA including exosomal RNA was purifiedfrom the depleted FBS, non-depleted FBS and a commercially available ready to go depleted FBS using Norgens Plasma/Serum Cell-Free Circulating DNA Purification Maxi Kit (Cat# 55800). Five different bovine microRNAs were assessed by RT-qPCR (miR-26a, miR-30a, miR-92a, miR-23a and miR-122). Three out of the five tested miRNA (miR-26a, miR-92a and miR-23a) didnt show any amplification in the FBS depleted using Norgen's FBS Exosome Depletion Kit I (Slurry Format) whereas the other two miRNAs (miR-30a and miR-122) showed very late Ct. values which appeared to be a primer dimer according to the melt curve.