ELF1 CUTANA CUT&RUN Antibody

CUTANA Compatible Antibodies meet the criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping.

Product Description

ELF1 is a member of the ETS (e26 transformation specific sequence) family of transcription factor proteins. ELF1 antibody produces CUT&RUN peaks above background primarily in intronic and promoter regions (Figure 1) that overlap with known ELF1 DNA-binding motifs (Figure 2). ELF1 is expressed in B and T cells and has been shown to be involved with the regulation of several T- and B-cell specific genes.                 

Figure 1: ELF1 peaks in CUT&RUN.
CUT&RUN was performed as described above. Peaks were called using MACS2. Heatmaps show ELF1 peaks relative to IgG negative control antibody in aligned rows ranked by intensity (top to bottom) and color such that red indicates high localized enrichment and blue denotes background signal (left). The number of peaks that fall into distinct classes of functionally annotated genomic regions are shown (right).

Figure 2: ELF1 transcription factor binding motif analysis in CUT&RUN
Homer analysis determined that the ELF1(ETS)/Jurkat-ELF1- ChIP-Seq(SRA014231)/Homer consensus motif, represented as a sequence logo position weight matrix, was significantly enriched under ELF1 CUT&RUN peaks (left). The number of ELF1 peaks containing the consensus motif from the left panel is represented by a Venn Diagram (right).

Every lot of a CUTANA Compatible antibody is tested in the indicated CUTANA approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein.